Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sri Kosuri
Date created: 2006-04-24 11:00:00
Date modified: 2015-05-08 01:14:54
T7.2 gene 4A/4B
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_Z0120_sequence (Version 1) |
Description
The full-length gene 4 protein is a 5??->3?? helicase of the E. coli DnaB family and a DNA primase. An in-frame internal start site within gene 4 results in a protein, gp4B, that lacks the N-terminal 63 amino acids of the 63 kDa gp4A, and consequently lacks primase activity. The primase recognition sites on ssDNA are 5??-G/TGGTC, and 5??-GTGTC.| Sequence Annotation | Location | Component / Role(s) |
| gene 4A gene 4B gene 4.1 gene 4.2 S4: possible promoter phi4c gat->gac 4.1 start cat->CAC 4.2 start ttt->TTC S4 -35 tta->CUG S4-10 codon shuffled (13 changes) phi4c gac...tac -> gat ... tat codon shuffle added ngoMIV | 1,1701 190,1701 71,193 1424,1762 574,637 1080,1114 70,72 1423,1425 586,588 610,612 1078,1116 4,39 1702,1707 | feature/cds CDS CDS feature/cds feature/cds CDS feature/cds CDS promoter feature/promoter feature/promoter promoter point_mutation feature/s_mutation feature/s_mutation point_mutation feature/s_mutation point_mutation point_mutation feature/s_mutation feature/s_mutation point_mutation feature/s_mutation point_mutation feature/mutation sequence_alteration |