Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Karmella Haynes
Date created: 2006-10-18 11:00:00
Date modified: 2015-05-08 01:14:55
Transcriptionally insulated high copy BioBrick plasmid
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | pSB1A7_sequence (Version 1) |
Description
pSB1A4 was constructed by adding double terminators ([BBa_B0015]) before the BioBrick prefix and after the BioBrick suffix in pSB1A3. This insulator blocks read-through transcription into BioBrick parts/ devices.Notes
The double terminators were assembled from small smaller overlapping ssDNA oligos. The outer-most EcoRI and PstI sites were mutated while the inner-most BioBrick cut sites were restored.Source
Based upon pSB1A3; Includes two copies of [BBa_B0015] assembled from DNA oligos| Sequence Annotation | Location | Component / Role(s) |
| suffix VF2 B0015 (rev) stem_loop stem_loop c > g mutated PstI E. coli his operon terminator stem_loop VR rep(pMB1) (rev) ampR (rev) end of terminator stem_loop mutated EcoRI c > g B0015 stem_loop stem_loop prefix | 1,20 2155,2174 24,152 38,57 98,141 153,153 153,158 159,229 167,201 294,313 398,1012 1172,2032 2231,2234 2244,2263 2273,2278 2278,2278 2279,2407 2290,2333 2374,2393 2411,2431 | feature/BioBrick engineered_region primer_binding_site feature/primer_binding feature/BioBrick engineered_region feature/stem_loop stem_loop feature/stem_loop stem_loop feature/mutation sequence_alteration feature/misc sequence_feature feature/misc sequence_feature stem_loop feature/stem_loop primer_binding_site feature/primer_binding feature/cds CDS CDS feature/cds feature/misc sequence_feature feature/stem_loop stem_loop feature/misc sequence_feature feature/mutation sequence_alteration feature/BioBrick engineered_region stem_loop feature/stem_loop stem_loop feature/stem_loop engineered_region feature/BioBrick |