BBa_M10041
BBa_M10041 Version 1 (Component)
{a~INP˃} | C-terminal displaying Ice Nucleation Protein

BBa_M10048
BBa_M10048 Version 1 (Component)
{˂Inp-repeats˃} Ice Nucleation Protein (Inp) Repeats

BBa_I714004
BBa_I714004 Version 1 (Component)
R100 relaxase ( nick the oriT of the R100 IncF-II conjugative plasmid )

BBa_K584028
BBa_K584028 Version 1 (Component)
pLac-Lux hybrid promoter + INP(Ice Nucleating Protein Generator)

BBa_K1352004
BBa_K1352004 Version 1 (Component)
Ice Nucleation Protein (INP) Yellow Florescent Protein (YFP) FLAG-tag

BBa_K1352003
BBa_K1352003 Version 1 (Component)
Ice Nucleation Protein with YFP and His-tag

BBa_K584023
BBa_K584023 Version 1 (Component)
Ice Neucleating Protein Generator (RBS + InaZ [K584021] + Terminator)

BBa_K584022
BBa_K584022 Version 1 (Component)
Ice Neucleating Protein Generator (RBS + InaZ [K584021] + Terminator)

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.