BBa_J70460
BBa_J70460 Version 1 (Component)
yfp RBS, {0,5;15,10} family member - B0032 simulator (forward oligo)

BBa_J70538
BBa_J70538 Version 1 (Component)
gfp RBS, {0,5;15,10} family member - B0034 simulator (forward oligo)

BBa_J70532
BBa_J70532 Version 1 (Component)
gfp RBS, {0,5;15,10} family member - B0031 simulator (forward oligo)

BBa_J70530
BBa_J70530 Version 1 (Component)
gfp RBS, {0,5;15,10} family member - B0030 simulator (forward oligo)

BBa_J70334
BBa_J70334 Version 1 (Component)
B1005.f.1: B1005 forward oligo part (single stranded)

BBa_J70440
BBa_J70440 Version 1 (Component)
yfp RBS, {0,5;15,10} family member - level 10 (forward oligo)

BBa_J70497
BBa_J70497 Version 1 (Component)
gfp RBS, {0,5;15,10} family member - B0029 simulator (forward oligo)

BBa_J70464
BBa_J70464 Version 1 (Component)
yfp RBS, {0,5;15,10} family member - B0034 simulator (forward oligo)

BBa_J70458
BBa_J70458 Version 1 (Component)
yfp RBS, {0,5;15,10} family member - B0031 simulator (forward oligo)

BBa_J70483
BBa_J70483 Version 1 (Component)
gfp RBS, {0,5;15,10} family member - level 10 (forward oligo)

BBa_J06601
BBa_J06601 Version 1 (Component)
Construction Intermediate: mCherry with forward terminator (E2060.B0015)

BioBrick-f
BBa_G1004 Version 1 (Component)
Forward BioBrick prefix primer for amplifying BioBrick parts (BioBrick-f)

BBa_J70449
BBa_J70449 Version 1 (Component)
yfp RBS, {0,5;15,10} family member - level 10000 (forward oligo)

BBa_J70446
BBa_J70446 Version 1 (Component)
yfp RBS, {0,5;15,10} family member - level 1000 (forward oligo)

BBa_K323080
BBa_K323080 Version 1 (Component)
CMV ATG cYFP link Zif268 link His stop - part of mammalian split/FRET system

BBa_K323029
BBa_K323029 Version 1 (Component)
CMV ATG cCFP link ZNF HIVC His stop - part of mammalian split/FRET system

BBa_J06600
BBa_J06600 Version 1 (Component)
Construction intermediate: Venus YFP with forward terminator (E2030.B0015)

BBa_K391002
BBa_K391002 Version 1 (Component)
Forward Primer to PCR agrCA (with RBS) from S. aureus

BBa_K315034
BBa_K315034 Version 1 (Component)
pBad + RBS + cre + pTet + lox5171 forward + RBS + RFP + lox5171 reverse

GG107
BBa_K2145102 Version 1 (Component)
GFP/RFP reporter (Forward/Reverse) separated by three dCas clamp sites

GG106
BBa_K2145101 Version 1 (Component)
GFP/RFP reporter (Reverse/Forward) separated by three dCas clamp sites

BBa_K497018
BBa_K497018 Version 1 (Component)
Forward primer Deinococcus radiodurans bacteriophytochrome gene for insertion after heme oxygenase

BBa_K1456009
BBa_K1456009 Version 1 (Component)
Glutathione Peroxidase (GPx) enzyme forward primer with restriction site and kozak sequence

BBa_J70342
BBa_J70342 Version 1 (Component)
J70315psri.f.1: J70315 psri forward part for pcr of S03621 (single stranded)

BBa_K1456007
BBa_K1456007 Version 1 (Component)
Superoxide dismutase-1 (SOD-1) Forward primer with restriction site and kozak sequence

BBa_K1456013
BBa_K1456013 Version 1 (Component)
Oxygen dependent degradation(ODD) domain from HIF-1a forward primer with restriction site

BBa_K1456011
BBa_K1456011 Version 1 (Component)
Human Tissue Plasminogen Activator (tPA) enzyme forward primer with restriction site and kozak seque

BBa_J06574
BBa_J06574 Version 1 (Component)
Construction intermediate: cI857 (Lamda II) with RBS and forward terminator (B0034.J06503.B0014)

BBa_K1456019
BBa_K1456019 Version 1 (Component)
Superoxide dismutase-1 (SOD-1) Forward primer-2 with restriction site and kozak sequence

BBa_K1456023
BBa_K1456023 Version 1 (Component)
Human Tissue Plasminogen Activator (tPA) enzyme forward primer-2 with restriction site and kozak seq

BBa_K1456021
BBa_K1456021 Version 1 (Component)
Glutathione Peroxidase-1 (GPx-1) enzyme forward primer-2 with restriction site and kozak sequence

BBa_K315045
BBa_K315045 Version 1 (Component)
pTet+loxP forward+RBS+RFP+loxN forward

BBa_K187318
BBa_K187318 Version 1 (Component)
ispU ORF, Forward Primer

BBa_K187059
BBa_K187059 Version 1 (Component)
kdsB ORF forward primer

BBa_K1616003
BBa_K1616003 Version 1 (Component)
VVD link to YC155 (YFP Cter split)

BBa_K1616004
BBa_K1616004 Version 1 (Component)
VVD linked to YN155 (YFP Nter split)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.