LacZ promoBBa_K119000 Version 1 (Component)Constitutive weak promoter of lacZ
BBa_K624064BBa_K624064 Version 1 (Component)nested_Inv R (inter-strain nested primer)
BBa_K624063BBa_K624063 Version 1 (Component)nested_Inv F (inter-strain nested primer)
BBa_K624065BBa_K624065 Version 1 (Component)nested_LLO F (inter-strain nested primer)
BBa_K624066BBa_K624066 Version 1 (Component)nested_LLO R (inter-strain nested primer)
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
BBa_K533012BBa_K533012 Version 1 (Component)safety version of pSB1C3 plasmid
Y taRNABBa_K606034 Version 1 (Component)Tyrosine amber supressor tRNA
BBa_K1319010BBa_K1319010 Version 1 (Component)Constitutive expression of K1319000
BBa_K1319012BBa_K1319012 Version 1 (Component)Constitutive expression of K1319002
BBa_K1465214BBa_K1465214 Version 1 (Component)RubisCO of Halothiobacillus neapolitanus and phosphoribulokinase A (prkA) under control of ptac
BBa_K1172566BBa_K1172566 Version 1 (Component)Combination of oprF under control of T7 promoter and gldA coding sequence
mdnA-myc-gBBa_K627006 Version 1 (Component)Fusion part of mdnA gene (from mdn-cluster) with myc-tag and gene III
CBDcipABBa_K1615111 Version 1 (Component)CBDcipA with N and C-terminal linker in RFC25
BBa_I728456BBa_I728456 Version 1 (Component)MerRT: Mercury-Inducible Promoter+RBS (MerR + part of MerT)
BBa_K784012BBa_K784012 Version 1 (Component)Spacer2 + RBS from theophylline riboswitch
BBa_K1314009BBa_K1314009 Version 1 (Component)amilCP, blue chromoprotein+ B0015+ part of mRFP
BBa_K1935002BBa_K1935002 Version 1 (Component)pUNC-119 : Neuron Specific Promoter of C.elegans
BBa_K337103BBa_K337103 Version 1 (Component)Fragment 6 of wt AAV9
BBa_K337059BBa_K337059 Version 1 (Component)Fragment 2 of wt AAV1
BBa_K337086BBa_K337086 Version 1 (Component)Fragment 5 of wt AAV6
BBa_K337098BBa_K337098 Version 1 (Component)Fragment 1 of wt AAV9
BBa_K337077BBa_K337077 Version 1 (Component)Fragment 4 of wt AAV5
BBa_K337087BBa_K337087 Version 1 (Component)Fragment 6 of wt AAV6
BBa_K337083BBa_K337083 Version 1 (Component)Fragment 2 of wt AAV6
BBa_K337072BBa_K337072 Version 1 (Component)Fragment 7 of wt AAV2
BBa_K1676103BBa_K1676103 Version 1 (Component)Lactate Permease of E. Coli
BBa_K337075BBa_K337075 Version 1 (Component)Fragment 2 of wt AAV5
BBa_K337074BBa_K337074 Version 1 (Component)Fragment 1 of wt AAV5
BBa_K351009BBa_K351009 Version 1 (Component)Front Part of modified FGFR
BBa_K337093BBa_K337093 Version 1 (Component)Fragment 4 of wt AAV8
BBa_K337095BBa_K337095 Version 1 (Component)Fragment 6 of wt AAV8
BBa_K337100BBa_K337100 Version 1 (Component)Fragment 3 of wt AAV9
BBa_K337065BBa_K337065 Version 1 (Component)Fragment 8 of wt AAV1
BBa_K337101BBa_K337101 Version 1 (Component)Fragment 4 of wt AAV9
BBa_K337066BBa_K337066 Version 1 (Component)Fragment 1 of wt AAV2
BBa_K337090BBa_K337090 Version 1 (Component)Fragment 1 of wt AAV8
BBa_K337069BBa_K337069 Version 1 (Component)Fragment 4 of wt AAV2
BBa_K337089BBa_K337089 Version 1 (Component)Fragment 8 of wt AAV6
BBa_K337097BBa_K337097 Version 1 (Component)Fragment 8 of wt AAV8
BBa_K809701BBa_K809701 Version 1 (Component)SP of ZIM17 + T7 RNAP
BBa_K337084BBa_K337084 Version 1 (Component)Fragment 3 of wt AAV6
BBa_K337096BBa_K337096 Version 1 (Component)Fragment 7 of wt AAV8
BBa_K337076BBa_K337076 Version 1 (Component)Fragment 3 of wt AAV5
BBa_K337081BBa_K337081 Version 1 (Component)Fragment 8 of wt AAV5
BBa_K337073BBa_K337073 Version 1 (Component)Fragment 8 of wt AAV2
BBa_K337062BBa_K337062 Version 1 (Component)Fragment 5 of wt AAV1
BBa_K337102BBa_K337102 Version 1 (Component)Fragment 5 of wt AAV9
BBa_K337105BBa_K337105 Version 1 (Component)Fragment 8 of wt AAV9
BBa_K648106BBa_K648106 Version 1 (Component)RecA1 with mutation of Arg243