Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
BBa_B0034BBa_B0034 Version 1 (Component)RBS (Elowitz 1999) -- defines RBS efficiency
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
BBa_B0030BBa_B0030 Version 1 (Component)RBS.1 (strong) -- modified from R. Weiss
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
BBa_B0032BBa_B0032 Version 1 (Component)RBS.3 (medium) -- derivative of BBa_0030
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
BBa_B0014BBa_B0014 Version 1 (Component)double terminator (B0012-B0011)
BBa_B0031BBa_B0031 Version 1 (Component)RBS.2 (weak) -- derivative of BBa_0030
BBa_B0033BBa_B0033 Version 1 (Component)RBS.4 (weaker) -- derivative of BBa_0030
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
BBa_E0430BBa_E0430 Version 1 (Component)EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)
B&TraJBBa_S03356 Version 1 (Component)B0034 connected to TraJ R
lacIBBa_Q04121 Version 1 (Component)QPI (B0034.C0012.B0015.R0011)
tetRBBa_Q04400 Version 1 (Component)QPI (B0034.C0040.B0015.R0040)
BBa_Q04510BBa_Q04510 Version 1 (Component)QPI (B0034.C0051.B0015.R0051)
OriTRBBa_J01003 Version 1 (Component)OriT-R (Origin of transfer for the R-plasmid nic region)
BBa_B0035BBa_B0035 Version 1 (Component)RBS (B0030 derivative)
BBa_K2066527BBa_K2066527 Version 1 (Component)B0034 with scar TACTAG
EYFPBBa_E0034 Version 1 (Component)enhanced yellow fluorescent protein derived from A. victoria GFP
B&TraJ FBBa_S03357 Version 1 (Component)B0034 connected to TraJ F
BBa_B0024BBa_B0024 Version 1 (Component)double terminator (B0012-B0011), reversed
BBa_S0100BBa_S0100 Version 1 (Component)B0034.C0012
BBa_E0422BBa_E0422 Version 1 (Component)ECFP (RBS+ LVA+ TERM) (B0034.E0022.B0015)
BBa_E0420BBa_E0420 Version 1 (Component)ECFP (RBS+ LVA- TERM) (B0034.E0020.B0015)
BBa_E0434BBa_E0434 Version 1 (Component)B0034.E0034.B0015
BBa_J44001BBa_J44001 Version 1 (Component)Reverse RBS (RBS<sub>rev</sub>) -- corresponds to BBa_B0030
BBa_S03518BBa_S03518 Version 1 (Component)B0034:C0040
CD4 signalBBa_I712009 Version 1 (Component)CD4 signal peptide; localizes to plasma membrane
BBa_S03154BBa_S03154 Version 1 (Component)B0034.C0078
BBa_J23089BBa_J23089 Version 1 (Component)RLambda traG knockout (oriTr-traJ::FRT, traG::CmR)
BBa_S03156BBa_S03156 Version 1 (Component)B0034.C0079
BBa_K208010BBa_K208010 Version 1 (Component)Lac Promoter (BBa_R0010) and RBS (B0034)
BBa_E0432BBa_E0432 Version 1 (Component)EYFP (RBS+ LVA+ TERM) (B0034.E0032.B0015)
BBa_J01078BBa_J01078 Version 1 (Component)R plasmid (x-OriT, x-TraJ)
BBa_B0054BBa_B0054 Version 1 (Component)Transcriptional terminator