GFPGFP Version 1 (Unknown)Organism: Cryptococcus neoformans serotype A H99S
iGEM Feature Librariesigem_feature_libraries_collection Version 1 (Unknown)A collection of iGEM feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).
Free Genes Feature Librariesfree_genes_feature_libraries_collection Version 1 (Unknown)A collection of Free Genes feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
ACS Synbio GenerationACS_Synbio_Generation Version 1 (Activity)Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
GFPBBa_E0040 Version 1 (Component)green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
BBa_B0031BBa_B0031 Version 1 (Component)RBS.2 (weak) -- derivative of BBa_0030
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
Amp CasBBa_I13800 Version 1 (Component)Ampicillin Resistance Cassette
ATGATG Version 1 (Component) GFP reportBBa_E0240 Version 1 (Component)GFP generator
BBa_J04450BBa_J04450 Version 1 (Component)RFP Coding Device
YFPBBa_E0032 Version 1 (Component)enhanced yellow fluorescent protein derived from A. victoria GFP
eyfpBBa_E0030 Version 1 (Component)enhanced yellow fluorescent protein derived from A. victoria GFP
GFP generaBBa_E0840 Version 1 (Component)GFP generator
BBa_K1399007BBa_K1399007 Version 1 (Component)GFP (mut3b) with SsrA-DAS+2 degradation tag
BBa_K1399005BBa_K1399005 Version 1 (Component)GFP (mut3b) with AAV-ssrA degradation tag
BBa_K1399004BBa_K1399004 Version 1 (Component)GFP (mut3b) with LVA-ssrA degradation tag
BBa_K1399008BBa_K1399008 Version 1 (Component)GFP (mut3b) with DAS-ssrA degradation tag
BBa_J04500BBa_J04500 Version 1 (Component)IPTG inducible promoter with RBS
BBa_K1399006BBa_K1399006 Version 1 (Component)GFP (mut3b) with LAA-ssrA degradation tag
ecfpBBa_E0020 Version 1 (Component)engineered cyan fluorescent protein derived from A. victoria GFP
BBa_J04031BBa_J04031 Version 1 (Component)GFP w/LVA tag
Scar25 ATGBBa_K1319106 Version 1 (Component)RFC 25 Translation Start Scar Sequence
BBa_K2066018BBa_K2066018 Version 1 (Component)UNS 2 Sequence, from Torella et al., 2013
ECFPBBa_E0022 Version 1 (Component)enhanced cyan fluorescent protein derived from A. victoria GFP
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
BBa_K1399003BBa_K1399003 Version 1 (Component)RFP from Discosoma striata (coral) with DAS-ssrA degradation tag
BBa_K844000BBa_K844000 Version 1 (Component)10x-Histidine (10x-His) Tag with double stop codon (TAATAA)
BBa_M0052BBa_M0052 Version 1 (Component)AANDENYADAS (moderately fast) SsrA degradation tag.
His-StopBBa_K133133 Version 1 (Component)His affinity tag-stop
RBS-RFPBBa_K093005 Version 1 (Component)RFP with RBS
BBa_K1399001BBa_K1399001 Version 1 (Component)RFP from Discosoma striata (coral) with LVA-ssrA degradation tag
mCherryBBa_J06504 Version 1 (Component)monomeric RFP optimized for bacteria
BBa_M0051BBa_M0051 Version 1 (Component)AANDENYNYADAS. (Fast) SsrA degradation tag.
BBa_K1399000BBa_K1399000 Version 1 (Component)RFP from Discosoma striata (coral) with AAV-ssrA degradation tag
RBS-GFP-TeBBa_I763020 Version 1 (Component)RBS-GFP (+LVA Tag)-Terminators
BBa_J04051BBa_J04051 Version 1 (Component)RFP with tag
LAABBa_M0050 Version 1 (Component)AANDENYALAA. (Very fast) SsrA degradation tag.
BBa_K1399002BBa_K1399002 Version 1 (Component)RFP from Discosoma striata (coral) with LAA-ssrA degradation tag (wt)