BBa_K106670BBa_K106670 Version 1 (Component)AarI AD acceptor, pRS315, 8X LexAOPs Cyc1P, Adht1
BBa_K106698BBa_K106698 Version 1 (Component)AarI AD acceptor vector (pRS315, 8X LexAOps Fig1P, Adh1t)
BBa_K106697BBa_K106697 Version 1 (Component)AarI AD acceptor vector (pRS315, Cyc1P, Adh1t-8XLexA Ops)
BBa_K896599BBa_K896599 Version 1 (Component)Strophurus rankini voucher AMS R140490 NADH dehydrogenase subunit 4 (ND4) gene, partial cds; tRNA-Hi
BBa_K1072003BBa_K1072003 Version 1 (Component)Lux pL controlled LuxR+Lux pR autoinduceing luxI(lva tag)+Lux pR controlled Ndh
BBa_M31114BBa_M31114 Version 1 (Component)M13, gene II from HpaI to the end [1-831bp]
BBa_K289009BBa_K289009 Version 1 (Component)pGADT7 is the AD Vector included with MATCHMAKER Two-Hybrid System 3.
BBa_K590044BBa_K590044 Version 1 (Component)ADC-PSB1A3-High constitutive
BBa_K590036BBa_K590036 Version 1 (Component)ADC-PSB3K3-High consititutive
BBa_K1072022BBa_K1072022 Version 1 (Component)Lux pI+RBS+LuxR+2TM+Lux pR+RBS+LuxI+2TM+Lux pR+RBS+GFP+2TM+Lux pR+RBS+Ndh+2TM
BBa_K809321BBa_K809321 Version 1 (Component)GAL promoter + YFP_SPtim21 + ADH1 terminator
BBa_K809308BBa_K809308 Version 1 (Component)GAL promoter + RFP_SPzim17 + ADH1 terminator
BBa_K1676088BBa_K1676088 Version 1 (Component)Mutant 12 of LDH and wildtype LDP
BBa_K1676091BBa_K1676091 Version 1 (Component)Mutant 15 of LDH and wildtype LDP
BBa_K1676086BBa_K1676086 Version 1 (Component)Mutant 10 of LDH and wildtype LDP
BBa_K1676080BBa_K1676080 Version 1 (Component)Mutant 4 of LDH and wildtype LDP
BBa_K809721BBa_K809721 Version 1 (Component)DLD3 promoter + kozak + hoiln(lambda phage) + ADH1 terminator
BBa_K809710BBa_K809710 Version 1 (Component)GAL promoter + kozak + ZIM17 + T7 RNAP + ADH1 terminator
BBa_K1154006BBa_K1154006 Version 1 (Component)Mating pheromone-induced IGPD and constitutive LDH expression in yeast
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.