Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
eyfpBBa_E0030 Version 1 (Component)enhanced yellow fluorescent protein derived from A. victoria GFP
ecfpBBa_E0020 Version 1 (Component)engineered cyan fluorescent protein derived from A. victoria GFP
ECFPBBa_E0022 Version 1 (Component)enhanced cyan fluorescent protein derived from A. victoria GFP
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
ecfpBBa_E0021 Version 1 (Component)ECFP (RBS+ LVA-)
EYFPBBa_E0034 Version 1 (Component)enhanced yellow fluorescent protein derived from A. victoria GFP
eyfpBBa_E0031 Version 1 (Component)EYFP (RBS+ LVA-)
BBa_E0430BBa_E0430 Version 1 (Component)EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)
GC Optimised Sequence PullGC_Optimised Version 1 (Activity)Used to indicate that a sequence was GC optimised after pulling from the source and before submission
BBa_E0422BBa_E0422 Version 1 (Component)ECFP (RBS+ LVA+ TERM) (B0034.E0022.B0015)
BBa_E0420BBa_E0420 Version 1 (Component)ECFP (RBS+ LVA- TERM) (B0034.E0020.B0015)
ECFPBBa_E0024 Version 1 (Component)enhanced cyan fluorescent protein derived from A. victoria GFP
BBa_K416008BBa_K416008 Version 1 (Component)Aga2 fused to eCFP
ecfpBBa_I15017 Version 1 (Component)B0032.EYFP
ecfpBBa_I15016 Version 1 (Component)B0032.ECFP
ECFPBBa_E0026 Version 1 (Component)enhanced cyan fluorescent protein derived from A. victoria GFP
OmpABBa_K103006 Version 1 (Component)OmpA outer membrane protein A fused to linker; displays proteins on cell surface
BBa_J44001BBa_J44001 Version 1 (Component)Reverse RBS (RBS<sub>rev</sub>) -- corresponds to BBa_B0030
CD4 signalBBa_I712009 Version 1 (Component)CD4 signal peptide; localizes to plasma membrane
EYFPBBa_E0036 Version 1 (Component)enhanced yellow fluorescent protein derived from A. victoria GFP
BBa_I15018BBa_I15018 Version 1 (Component)polycistronic ECFP+EYFP under B0031
BBa_I15015BBa_I15015 Version 1 (Component)polycistronic ECFP+EYFP under B0032
BBa_E0432BBa_E0432 Version 1 (Component)EYFP (RBS+ LVA+ TERM) (B0034.E0032.B0015)
BBa_K1321342BBa_K1321342 Version 1 (Component)sfGFP fused to CBDcex in RFC 25
BBa_K309020BBa_K309020 Version 1 (Component)eCFP (for use in C. elegans chassis)
aGalBBa_K1483002 Version 1 (Component)α-Galactosidase
BBa_K1321341BBa_K1321341 Version 1 (Component)sfGFP fused to CBDclos in RFC 25
BBa_K1321122BBa_K1321122 Version 1 (Component)Smt fused to CBDcex
BBa_K1321349BBa_K1321349 Version 1 (Component)CBDcex fused to sfGFP in RFC 25
BBa_K1321343BBa_K1321343 Version 1 (Component)NiBP fused to CBDclos in RFC 25
BBa_K1321344BBa_K1321344 Version 1 (Component)NiBP fused to CBDcex in RFC 25
BBa_K1321151BBa_K1321151 Version 1 (Component)metallothionein Fmt fused to CBDclos
BBa_K1321121BBa_K1321121 Version 1 (Component)Smt metallothionein fused to CBDclos
BBa_K1321152BBa_K1321152 Version 1 (Component)metallothionein Fmt fused to CBDcex
BBa_K1321154BBa_K1321154 Version 1 (Component)CBDclos fused to metallothionein Fmt
BBa_K1365169BBa_K1365169 Version 1 (Component)signal sequence from USP45 fused to aiiA