ACS Synbio GenerationACS_Synbio_Generation Version 1 (Activity)Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
ATGATG Version 1 (Component) Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
Scar25 ATGBBa_K1319106 Version 1 (Component)RFC 25 Translation Start Scar Sequence
AraBBa_K808000 Version 1 (Component)araC-Pbad - Arabinose inducible regulatory promoter/repressor unit
rhIIBBa_C0070 Version 1 (Component)autoinducer synthetase for N-butyryl-HSL (BHL) and HHL
GC Optimised Sequence PullGC_Optimised Version 1 (Activity)Used to indicate that a sequence was GC optimised after pulling from the source and before submission
RFC105 ABBa_K1362414 Version 1 (Component)N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A
BBa_K1321340BBa_K1321340 Version 1 (Component)Double CBD (dCBD) with N-terminal linker
ArgRBO_11438 Version 1 (Component) NpuDnaE(N)BBa_K1362400 Version 1 (Component)NpuDnaE N-Intein cloning piece
BBa_K249008BBa_K249008 Version 1 (Component)YFP with N-terminal Arganine Tag
LacIBBa_K143033 Version 1 (Component)LacI (Lva<sup>-</sup>, N-terminal deletion) regulatory protein
BBa_K331030BBa_K331030 Version 1 (Component)Tet Repressible N-terminal Arg Tagged EYFP (no terminator)
BBa_K1423000BBa_K1423000 Version 1 (Component)BCLA N-terminal Domain
BBa_J78007BBa_J78007 Version 1 (Component)Pnir-Arg[N]
BBa_J78005BBa_J78005 Version 1 (Component)Lac-Arg[N]
G-His6(N)BBa_K1362430 Version 1 (Component)C-terminal His6 (N-Intein)
BBa_I759001BBa_I759001 Version 1 (Component)pTet-spacer-N
1NpuE(N*)BBa_K1362102 Version 1 (Component)RBS + NpuDnaE N-intein RFC[105] non-splicing control construct (with His6)
BBa_K809601BBa_K809601 Version 1 (Component)GAL promoter (kozak) without start codon ATG
RFC105 NNBBa_K1362415 Version 1 (Component)N-terminal splicing overhang TGCT=Cys+1/3 Leu|Ile RFC[105] overhang NN
R0011-NBBa_K1725080 Version 1 (Component)Promoter (lacI regulated, lambda pL hybrid) with extra Nhe I site
N-carrierBBa_K1362053 Version 1 (Component)Carrier for RFC[105] N-terminal inserts
BBa_K315007BBa_K315007 Version 1 (Component)Lox N forward
Dsb-Art175BBa_K1659002 Version 1 (Component)Artilysin Art-175 fused at N-terminal with DsbA signal peptide
lacZ aBBa_E0033 Version 1 (Component)LacZ alpha fragment; complements matching N-terminal deletion mutant (lacZ-omega)
TerDnaE3(NBBa_K1362130 Version 1 (Component)RBS + TerDnaE3 N-intein RFC[105] assembly construct (with His6)
SspDnaB(N*BBa_K1362142 Version 1 (Component)RBS + SspDnaB N-intein RFC[105] non-splicing control construct (with His6)
SspDnaB(N)BBa_K1362140 Version 1 (Component)RBS + SspDnaB N-intein RFC[105] assembly construct (with His6)
SspDnaX(N)BBa_K1362110 Version 1 (Component)RBS + SspDnaX N-intein RFC[105] assembly construct (with His6)
SspGyrB(N)BBa_K1362120 Version 1 (Component)RBS + SspGyrB N-intein RFC[105] assembly construct (with His6)
His-ScaffoBBa_K1602027 Version 1 (Component)Protein scaffold with N-terminal HisTag
NAGABBa_K1483000 Version 1 (Component)α-N-Acetylgalactosamindase
BBa_K801072BBa_K801072 Version 1 (Component)3,7-dimethylxanthine N-methyltransferase CaDXMT1-strep
BBa_K801071BBa_K801071 Version 1 (Component)7-methylxanthine N-methyltransferase CaMXMT1-strep
BBa_K331001BBa_K331001 Version 1 (Component)ECFP with N-terminal Arginine Tag