BBa_K1660005
BBa_K1660005 Version 1 (Component)
RFP controlled by the PompR promoter

YFP_SPtom4
BBa_K809316 Version 1 (Component)
YFP + signal peptide of Tom40

BBa_J23080
BBa_J23080 Version 1 (Component)
b0034 derived RBS to match spacing of J01122

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1431301
BBa_K1431301 Version 1 (Component)
TRE-3G promoter+SV40 PolyA, an ideal controller of mammalian gene expression with Tet-On 3G protein

BBa_K1075041
BBa_K1075041 Version 1 (Component)
RBS32-MazE-(Ec)ssrA(DAS+4)

BBa_K1001758
BBa_K1001758 Version 1 (Component)
AraC coding upstream of Lux promoter upstream of GFP

BBa_K2044001
BBa_K2044001 Version 1 (Component)
Based on our project, <2-4-8> is a feasible pathway from Site No. 2 to Site No. 8

BBa_K814008
BBa_K814008 Version 1 (Component)
ScyB (leucine dehydrogenase) generator

DR-Bph
BBa_K646002 Version 1 (Component)
Deinococcus radiodurans bacteriophytochrome

BBa_K1053100
BBa_K1053100 Version 1 (Component)
RNA scaffold

BBa_I759038
BBa_I759038 Version 1 (Component)
Ptet_cis4_YFP

BBa_K076004
BBa_K076004 Version 1 (Component)
EYFP

BBa_S05273
BBa_S05273 Version 1 (Component)
Leucine Zipper with flexible linker

BBa_I11013
BBa_I11013 Version 1 (Component)
Modification of biobricks part BBa_B0015

SEGA023
SEGA023 Version 1 (Component)
natural linear DNA.

SEGA012
SEGA012 Version 1 (Component)
natural linear DNA.

SEGA025
SEGA025 Version 1 (Component)
natural linear DNA.

SEGA020
SEGA020 Version 1 (Component)
natural linear DNA.

SEGA080
SEGA080 Version 1 (Component)
natural linear DNA.

SEGA082
SEGA082 Version 1 (Component)
natural linear DNA.

SEGA059
SEGA059 Version 1 (Component)
natural linear DNA.

SEGA092
SEGA092 Version 1 (Component)
natural linear DNA.

SEGA065
SEGA065 Version 1 (Component)
natural linear DNA.

SEGA095
SEGA095 Version 1 (Component)
natural linear DNA.

SEGA064
SEGA064 Version 1 (Component)
natural linear DNA.

SEGA075
SEGA075 Version 1 (Component)
natural linear DNA.

Strong+Imm
BBa_K320006 Version 1 (Component)
Constitutive expression of Immunotoxin

BBa_K1375011
BBa_K1375011 Version 1 (Component)
Plac+RBS+LuxI+DT

BBa_K1058003
BBa_K1058003 Version 1 (Component)
Promoter of penicillinase blaZ as well as promoter of blaR1

BBa_S05157
BBa_S05157 Version 1 (Component)
GFP+I702

BBa_K1583013
BBa_K1583013 Version 1 (Component)
CsgA missing the start codon for fusion protein

BBa_S04437
BBa_S04437 Version 1 (Component)
R0040:J61046

BBa_K189061
BBa_K189061 Version 1 (Component)
Gp16 of Bacteriophage Mu

BBa_K835002
BBa_K835002 Version 1 (Component)
cytoplasmic region of tar

BBa_K189065
BBa_K189065 Version 1 (Component)
E18 of Bacteriophage Mu

BBa_K189068
BBa_K189068 Version 1 (Component)
C of Bacteriophage Mu

BBa_J100020
BBa_J100020 Version 1 (Component)
Second Half of AspC

BBa_K189052
BBa_K189052 Version 1 (Component)
GP7 of Bacteriophage Mu

BBa_K189050
BBa_K189050 Version 1 (Component)
Kil of Bacteriophage Mu

BBa_M30050
BBa_M30050 Version 1 (Component)
Refactoring of M13K07 genome

BBa_K189075
BBa_K189075 Version 1 (Component)
Gp28 of Bacteriophage Mu

BBa_K189056
BBa_K189056 Version 1 (Component)
Gp11 of Bacteriophage Mu

BBa_K189062
BBa_K189062 Version 1 (Component)
Gp16 of Bacteriophage Mu

BBa_K189057
BBa_K189057 Version 1 (Component)
Gp12 of Bacteriophage Mu

BBa_J61052
BBa_J61052 Version 1 (Component)
[neuS1] of K1 ORF

BBa_K189051
BBa_K189051 Version 1 (Component)
Gp6 of Bacteriophage Mu

BBa_K189070
BBa_K189070 Version 1 (Component)
Gp23 of Bacteriophage Mu

BBa_K189066
BBa_K189066 Version 1 (Component)
E19 of Bacteriophage Mu

BBa_I714002
BBa_I714002 Version 1 (Component)
traI of F plasmid