BBa_K1075042
BBa_K1075042 Version 1 (Component)
RBS32-MazE-(Ec)ssrA(DAS+4)-TT

BBa_K1491035
BBa_K1491035 Version 1 (Component)
Constitutive ER Intein T7 RNAP with YFPH

BBa_K1058008
BBa_K1058008 Version 1 (Component)
Cr(VI) sensor with egfp(PchrB+chrB+egfp)

BBa_K1462520
BBa_K1462520 Version 1 (Component)
GAL1+YFP+SH3-lig+ADH1+GAL1+GFP+PDZ-lig+ADH1+GAL1+BFP+GBD-lig+ADH1

BBa_K1462400
BBa_K1462400 Version 1 (Component)
pGAL1+PRK+GBD-ligand+ADH1+pTDH3+RuBisCo+SH3-ligand+ADH1+pTDH3+CA+PDZ-ligand+ADH1

BBa_K1491029
BBa_K1491029 Version 1 (Component)
J23115-B0034-T7 RNA Polymerase with ER Intein

BBa_K1491036
BBa_K1491036 Version 1 (Component)
Constitutive Dead ER Intein T7 RNAP with YFPH

BBa_K1462420
BBa_K1462420 Version 1 (Component)
pTEF2+GroES+ADH1+pTEF2+GroEL+ADH1+pGAL1+PRK+GBD-ligand+ADH1+pTDH3+RuBisCo+SH3-ligand+ADH1

BBa_I719004
BBa_I719004 Version 1 (Component)
ech gene

BBa_K1075026
BBa_K1075026 Version 1 (Component)
AraC-pBad-RBS34-mCherry-(Ec)ssrA(DAS+4)-TT

BBa_K1491031
BBa_K1491031 Version 1 (Component)
J23115-B0034-T7 RNA Polymerase with Dead ER Intein

BBa_K1462410
BBa_K1462410 Version 1 (Component)
pTEF2+GroES+tADH1+pTEF2+GroEL+tADH1+pGAL1+PRK+GBD-ligand+tADH1+pTDH3+RuBisCo+SH3-ligand+tADH1+pTDH3+

BBa_K737000
BBa_K737000 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

BBa_K238028
BBa_K238028 Version 1 (Component)
ech with RBS

BBa_K1075027
BBa_K1075027 Version 1 (Component)
SplitSspB(Rapamycin inducable)-pLac-RBS34-mCherry-(Ec)ssrA(DAS+4)-TT

BBa_K737001
BBa_K737001 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

BBa_I742011
BBa_I742011 Version 1 (Component)
ech (feruloyl hydratase/aldolase; vanillin biosynthesis)

BBa_I719001
BBa_I719001 Version 1 (Component)
ech and fcs genes

BBa_I719011
BBa_I719011 Version 1 (Component)
ech and fcs genes

BBa_K1075048
BBa_K1075048 Version 1 (Component)
AraC-pBAD-RBS32-mazF-TT-pLac-RBS32-mazE-(Ec)ssrA(DAS+4)-TT

BBa_I754000
BBa_I754000 Version 1 (Component)
Feruloyl CoA Hyratase for vanillin biosynthesis-ech

IBMc380
IBMc380 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86(1-39)-LD-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(40-154)-S-mCherry*(193-236)-H6-T

BBa_I754001
BBa_I754001 Version 1 (Component)
ech protein generator for vanillin biosynthesis

BBa_I769106
BBa_I769106 Version 1 (Component)
ech protein generator for vanillin biosynthesis

BBa_I735000
BBa_I735000 Version 1 (Component)
ech gene coding sequence for feruoyl CoA hydratese

BBa_I718001
BBa_I718001 Version 1 (Component)
ech My new test generator part Feruloyl CoA hydratase for vanilin

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.