BBa_K737003
BBa_K737003 Version 1 (Component)
This part is polyphosphate kinase.

BBa_K737016
BBa_K737016 Version 1 (Component)
This part conteins gvpA protein's coding sequence.

BBa_K737017
BBa_K737017 Version 1 (Component)
This part conteins gvpC-20psi protein

BBa_K737022
BBa_K737022 Version 1 (Component)
This part is gvpC-20 coding gene with GFP mut 3.1 fusion gene

BBa_K863011
BBa_K863011 Version 1 (Component)
tthl laccase from Thermus thermophilus

BBa_K863012
BBa_K863012 Version 1 (Component)
tthl laccase from T. thermophilus with constitutive promoter J23100, RBS and His-tag

BBa_K750005
BBa_K750005 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.01 strength)

CBDcex
BBa_K863101 Version 1 (Component)
Cellulose binding Domain of Cellulomonas Fimi Exoglucanse (Freiburg-Standard)

BBa_K737058
BBa_K737058 Version 1 (Component)
Spot42 is a multitarget small RNA that mediates the discoordinate expression of the E.coli galactose

CBDclos
BBa_K863111 Version 1 (Component)
Cellulose binding domain of C. cellulovorans cellulose binding protein gene (Freiburg-Standard)

BBa_K737033
BBa_K737033 Version 1 (Component)
galK is the leader sequence of galactokinase (GalK)

BBa_K739003
BBa_K739003 Version 1 (Component)
promoter of raxRH + RBS + mRFP

BBa_K873003
BBa_K873003 Version 1 (Component)
conjugative transfer of RFG

BBa_K737048
BBa_K737048 Version 1 (Component)
The leading sequence involved in the nanC transcript which can act as the target of the sRNA, spot42

BBa_K737049
BBa_K737049 Version 1 (Component)
The leading sequence involved in the YtfJ transcript which can act as the target of the sRNA, spot42

BBa_K737050
BBa_K737050 Version 1 (Component)
The leading sequence involved in the SrlA transcript which can act as the target of the sRNA, spot42

BBa_K739004
BBa_K739004 Version 1 (Component)
Ax21 protein - signaling part

BBa_K777101
BBa_K777101 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23100

P-alsT
BBa_K818300 Version 1 (Component)
Promoter alsT, repressed by TnrA during conversion of NH4 to Glutamine in B. subtilis.

BBa_K777102
BBa_K777102 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23104

BBa_K777103
BBa_K777103 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23105

BBa_K777104
BBa_K777104 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23106

BBa_K777105
BBa_K777105 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23109

BBa_K777106
BBa_K777106 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23112

BBa_K777107
BBa_K777107 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23113

BBa_K777108
BBa_K777108 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23114

BBa_K769002
BBa_K769002 Version 1 (Component)
Kinase domain of EnvZ

CBDcex(T7)
BBa_K863102 Version 1 (Component)
Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)

BBa_K750008
BBa_K750008 Version 1 (Component)
Quorum sensing system based on LuxI and LuxR to control the expression of parts behind

BBa_K774002
BBa_K774002 Version 1 (Component)
Comparator Circuit Part 1

BBa_K774003
BBa_K774003 Version 1 (Component)
Comparator Circuit Part 2

BBa_K102906
BBa_K102906 Version 1 (Component)
TA8 gate from synthetic algorithm v1.1

BBa_K102907
BBa_K102907 Version 1 (Component)
TA9 gate from synthetic algorithm v1.1

BBa_K102908
BBa_K102908 Version 1 (Component)
TA10 gate from synthetic algorithm v1.1

BBa_K102909
BBa_K102909 Version 1 (Component)
TA11 gate from synthetic algorithm v1.1

BBa_K102910
BBa_K102910 Version 1 (Component)
TA12 gate from synthetic algorithm v1.1

BBa_K098984
BBa_K098984 Version 1 (Component)
lac inducible mtrB (high expression of QPI)

BBa_K098983
BBa_K098983 Version 1 (Component)
lac inducible mtrB (low expression of QPI)

BBa_K157002
BBa_K157002 Version 1 (Component)
Transmembrane region of the EGF-Receptor (ErbB-1)

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Cello E. Coli Parts
Eco1C1G1T1_collection Version 1 (Collection)
These are the Cello parts for E. Coli circuits

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K102911
BBa_K102911 Version 1 (Component)
TA13 gate from synthetic algorithm v1.2

Q04510+RFP
BBa_K131018 Version 1 (Component)
Intermediate for the Response circuit

BBa_K102905
BBa_K102905 Version 1 (Component)
TA7 gate from synthetic algorithm v1.1

lexA
BBa_K165020 Version 1 (Component)
LexA Synthetic Activator (untagged)

BBa_K165021
BBa_K165021 Version 1 (Component)
Kozak + LexA Synthetic Activator (untagged)

BBa_K165022
BBa_K165022 Version 1 (Component)
Zif268-HIV synthetic activator (untagged)

BBa_K165023
BBa_K165023 Version 1 (Component)
Kozak + Zif268-HIV synthetic activator (untagged)