BBa_I13667BBa_I13667 Version 1 (Component)Cascaded Lac (elowitz) and Tet (medium) QPIs with constitutive promoter as input
BBa_I13631BBa_I13631 Version 1 (Component)Cascaded Tet (weak) and Lac (medium) QPIs with double terminator as input
BBa_I13657BBa_I13657 Version 1 (Component)Cascaded Tet (elowitz) and Lac (medium) QPIs with constitutive promoter as input
BBa_I13637BBa_I13637 Version 1 (Component)Cascaded Tet (elowitz) and Lac (medium) QPIs with double terminator as input
BBa_K1947023BBa_K1947023 Version 1 (Component)This part serves as a catch system expressed in <i>E. coli.
BBa_K880003BBa_K880003 Version 1 (Component)Asymmetrically digestible reporter, same orientation of inverted repeats as K137058 (external sites
Plac-LacY-BBa_I763032 Version 1 (Component)LacY coding device switched on by IPTG with GFP as a reporter
M13 -47BBa_K176059 Version 1 (Component)M13 -47 general primer as a reverse primer binds to 5prime terminal of lacZ
pLacIQ1-RBBBa_K193406 Version 1 (Component)the coding is the same as BBa_193404 the back bone is changed onto pSB6A1
BBa_K737050BBa_K737050 Version 1 (Component)The leading sequence involved in the SrlA transcript which can act as the target of the sRNA, spot42
BBa_K1412089BBa_K1412089 Version 1 (Component)Riboregulator which combines crRNA and RBS acting as a lock to the gene cuicuit
BBa_J100198BBa_J100198 Version 1 (Component)J100198 contains the fitness gene tester construct (J100199) with thymidylate synthase as the fitnes
DGATBBa_K836002 Version 1 (Component)O-acyltransferase WSD from Acinetobacter sp. (codon usage optimized for R. opacus) as used
BBa_K1230003BBa_K1230003 Version 1 (Component)RBS-GFP-Terminator with LL-37 antimicrobial peptide with modified codon usage for E. coli as prefix
BBa_J70652BBa_J70652 Version 1 (Component)This tests adding an internal strep tag linker to a protein using J70590 as a te
BBa_J70653BBa_J70653 Version 1 (Component)This tests adding an internal strep tag linker to a protein using J70590 as a te
BBa_K2097000BBa_K2097000 Version 1 (Component)CpxR binding site attached to a yellow-green color protein (YGCP) acts as a neutral pH indicator.
BBa_K1362061BBa_K1362061 Version 1 (Component)N-terminal chitin binding domain with triglycine linker and His-tag as RFC[105] insert
BBa_K1778005BBa_K1778005 Version 1 (Component)eGFP:enhanced Green Fluorescent Protein. It???s the mutant of GFP. It is widely used as report gene
CMV + GFPBBa_K1852000 Version 1 (Component)GFP + CMV Promoter used for expression in oocyte cells. This part was planned to be used as a report
BBa_K603002BBa_K603002 Version 1 (Component)same as BBa_K603000 except in pSB1C3 vector
pCMV-ECFP-BBa_I763023 Version 1 (Component)LacI coding device with ECFP as a reporter regulated by pCMV
Bacillus subtilis Collectionbsu_collection Version 1 (Collection)This collection includes information about promoters, operators, CDSs and proteins from Bacillus subtilis. Functional interactions such as transcriptional activation and repression, protein production and various protein-protein interactions are also included.
BBa_J24823BBa_J24823 Version 1 (Component)same as J24819 but with ACCACC Euk RBS removed and problem solved via J24822 removed
iGEM Parts Registryigem_collection Version 1 (Collection)The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.