BBa_K2029015BBa_K2029015 Version 1 (Component)pSBbs1AK1 (replicative Bacillus subtilis expression vector)
BBa_K1159002BBa_K1159002 Version 1 (Component)Laccase from <i>Bacillus pumilus</i> in RFC[25]
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
BBa_K1820011BBa_K1820011 Version 1 (Component)RBS + sfGFP(Bs) + terminator
BBa_I13001BBa_I13001 Version 1 (Component)RBS B0030 w/ YFP (-LVA) TT
BBa_I742156BBa_I742156 Version 1 (Component)crtZ native rbs
iGEM 2019 DistributioniGEM2019Distribution_collection Version 1 (Collection)Distribution of parts for the 2019 iGEM competition
iGEM 2018 DistributioniGEM2018Distribution_collection Version 1 (Collection)Distribution of parts for the 2018 iGEM competition
iGEM DistributionsiGEMDistributions_collection Version 1 (Collection)Part distributions for the iGEM competition
iGEM Parts Registryigem_collection Version 1 (Collection)The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.
BBa_K1033929BBa_K1033929 Version 1 (Component)aeBlue, blue chromoprotein (incl RBS)
BBa_K863200BBa_K863200 Version 1 (Component)5' UTR site of alcohol oxidase 1 gene (aox1)
BBa_K1955003BBa_K1955003 Version 1 (Component)Leishmania-5 prime UTR-Hygromycin
BBa_K390057BBa_K390057 Version 1 (Component)pilA1 promoter, 5'UTR, and RBS with GFP
BBa_K654093BBa_K654093 Version 1 (Component)Synechocystis psbA2 5'UTR Native RBS + Renilla Luciferase
BBa_K390058BBa_K390058 Version 1 (Component)pixJI promoter, 5'UTR, and RBS with GFP
BBa_K390050BBa_K390050 Version 1 (Component)psbA2 promoter, 5'UTR, and RBS with GFP
BBa_K390060BBa_K390060 Version 1 (Component)sll0837 promoter, 5'UTR, and RBS with GFP
BBa_K390047BBa_K390047 Version 1 (Component)hspA promoter, 5'UTR, and RBS with GFP
BBa_K390052BBa_K390052 Version 1 (Component)glnBP2 promoter, 5'UTR, and RBS with GFP
BBa_K390059BBa_K390059 Version 1 (Component)sigF promoter, 5'UTR, and RBS with GFP
BBa_K390056BBa_K390056 Version 1 (Component)sigE promoter, 5'UTR, and RBS with GFP
BBa_K390054BBa_K390054 Version 1 (Component)psbD2 promoter, 5'UTR, and RBS with GFP
BBa_K390055BBa_K390055 Version 1 (Component)sigB promoter, 5'UTR, and RBS with GFP
BBa_K654094BBa_K654094 Version 1 (Component)Synechocystis psbA2 5'UTR Consensus RBS + Renilla Luciferase
BBa_K390053BBa_K390053 Version 1 (Component)lrtA promoter, 5'UTR, and RBS with GFP
BBa_K390051BBa_K390051 Version 1 (Component)sigA promoter, 5'UTR, and RBS with GFP
BBa_K1950000BBa_K1950000 Version 1 (Component)trc promoter + RBS
BBa_K1336002BBa_K1336002 Version 1 (Component)Spore coat protein, laccase, from Bacillus subtilis
BBa_J70029BBa_J70029 Version 1 (Component)RBS + mCherry gene, optimized for expression in Me. florum and E. coli
RBS-pcyABBa_K193402 Version 1 (Component)RBS is located upstream of pcyA
BBa_K174011BBa_K174011 Version 1 (Component)IPTG inducable kinA Bacillus sporulation trigger
BBa_K174009BBa_K174009 Version 1 (Component)CotC, Bacillus subtilis spore coat protein
BBa_M1528BBa_M1528 Version 1 (Component)RBS + HlyB + RBS + HlyD
RBS-cIBBa_K093006 Version 1 (Component)cI repressor with RBS
BBa_K1520504BBa_K1520504 Version 1 (Component)Rbs-luxI(scarless)
BBa_K2017002BBa_K2017002 Version 1 (Component)35s promoter + 5' region
BBa_K390030BBa_K390030 Version 1 (Component)psbA2 promoter, 5'UTR and native RBS, GFP
BBa_K976010BBa_K976010 Version 1 (Component)psbA2 promoter, 5'UTR, and RBS + Firefly Luciferase
RBS-CI-RBSBBa_K079011 Version 1 (Component)CI protein with RBS and GFP reporter protein
BBa_K1491005BBa_K1491005 Version 1 (Component)RBS-SuperYFP2-His
BBa_J64971BBa_J64971 Version 1 (Component)rocF coding sequence from bacillus subtilis subsp. subtilis str. 168
BBa_K741006BBa_K741006 Version 1 (Component)RBS-crogfp-T
BBa_K332033BBa_K332033 Version 1 (Component)37℃ induced RBS + tetR + double terminator
rbs+crtYBBa_I742155 Version 1 (Component)crtY (lycopene cyclase) with native rbs