BBa_K1551000BBa_K1551000 Version 1 (Component)To generate delta-4 fatty acid Desaturase so as to synthesize the DHA.
BBa_K136048BBa_K136048 Version 1 (Component)RBS - lasR - double terminator
BBa_K091180BBa_K091180 Version 1 (Component)pLac-RBS-LasR-TT
BBa_K880003BBa_K880003 Version 1 (Component)Asymmetrically digestible reporter, same orientation of inverted repeats as K137058 (external sites
BBa_K2180012BBa_K2180012 Version 1 (Component)Promoter PlepA +FUR regulatory sequence + RBS + LacI-LVA + Terminator + Pigment + RBS + Operateur La
BBa_K358000BBa_K358000 Version 1 (Component)Lac promoter with GFP
BBa_K876035BBa_K876035 Version 1 (Component)pLuxR-LasI-RBS-RFP
BBa_K136033BBa_K136033 Version 1 (Component)RBS lasI - gfp tripart
Plac-LacY-BBa_I763032 Version 1 (Component)LacY coding device switched on by IPTG with GFP as a reporter
BBa_K1072007BBa_K1072007 Version 1 (Component)Lux pI+RBS+LuxR+2TM+Lux pR+RBS+LuxI+2TM+Lux pR+RBS+GFP+2TM+Lux pR+RBS+α-ALS+2TM
M13 -47BBa_K176059 Version 1 (Component)M13 -47 general primer as a reverse primer binds to 5prime terminal of lacZ
pLacIQ1-RBBBa_K193406 Version 1 (Component)the coding is the same as BBa_193404 the back bone is changed onto pSB6A1
BBa_M36561BBa_M36561 Version 1 (Component)This terminator is a general terminator of transcription. It forms a stem loop which stops transcrip
BBa_K136034BBa_K136034 Version 1 (Component)RBS lasI - gfp tripart
CP1LacPI2BBa_K418002 Version 1 (Component)IPTG inducible Lac promoter cassette
BBa_K737050BBa_K737050 Version 1 (Component)The leading sequence involved in the SrlA transcript which can act as the target of the sRNA, spot42
BBa_K1412089BBa_K1412089 Version 1 (Component)Riboregulator which combines crRNA and RBS acting as a lock to the gene cuicuit
BBa_J100198BBa_J100198 Version 1 (Component)J100198 contains the fitness gene tester construct (J100199) with thymidylate synthase as the fitnes
BBa_K136056BBa_K136056 Version 1 (Component)RBS - lasI - ECFP Tripart (+LVA)
DGATBBa_K836002 Version 1 (Component)O-acyltransferase WSD from Acinetobacter sp. (codon usage optimized for R. opacus) as used
BBa_K1230003BBa_K1230003 Version 1 (Component)RBS-GFP-Terminator with LL-37 antimicrobial peptide with modified codon usage for E. coli as prefix
BBa_J70652BBa_J70652 Version 1 (Component)This tests adding an internal strep tag linker to a protein using J70590 as a te
BBa_J06101BBa_J06101 Version 1 (Component)Tet repressible lasI generator ( LVA+ )
BBa_K648102BBa_K648102 Version 1 (Component)RecA with mutation of Lys 286
BBa_K794001BBa_K794001 Version 1 (Component)CSP(derived from E. coli) - lasI
BBa_J70653BBa_J70653 Version 1 (Component)This tests adding an internal strep tag linker to a protein using J70590 as a te
BBa_K2097000BBa_K2097000 Version 1 (Component)CpxR binding site attached to a yellow-green color protein (YGCP) acts as a neutral pH indicator.
BBa_I715071BBa_I715071 Version 1 (Component)Lac promoter with Ribosomal Binding Site
BBa_K136023BBa_K136023 Version 1 (Component)strong promoter - RBS - lasR - double terminator
BBa_K136041BBa_K136041 Version 1 (Component)Strongest RBS - lasR activator with LVA
BBa_K1362061BBa_K1362061 Version 1 (Component)N-terminal chitin binding domain with triglycine linker and His-tag as RFC[105] insert
BBa_K1778005BBa_K1778005 Version 1 (Component)eGFP:enhanced Green Fluorescent Protein. It???s the mutant of GFP. It is widely used as report gene
BBa_K136052BBa_K136052 Version 1 (Component)lasR activator + LVA (C0079) - Double Terminator (B0015)
BBa_K590046BBa_K590046 Version 1 (Component)AAR-PSB3K3-Lac Inducible w/o LacI
BBa_K418001BBa_K418001 Version 1 (Component)From partsregistry.org IPTG inducible Lac promoter cassette
BBa_K584011BBa_K584011 Version 1 (Component)Lac-Lux hybrid promotor + CrtEBI + CI repressor + INP
BBa_K1222004BBa_K1222004 Version 1 (Component)pCam(T7 promoter+lac operator+CamR antisense 2+T7 terminator)
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
BBa_J22121BBa_J22121 Version 1 (Component)Lac Y gene under the rec A(SOS) promoter in plasmid pSB2K3
BBa_K603002BBa_K603002 Version 1 (Component)same as BBa_K603000 except in pSB1C3 vector
pCMV-ECFP-BBa_I763023 Version 1 (Component)LacI coding device with ECFP as a reporter regulated by pCMV
Bacillus subtilis Collectionbsu_collection Version 1 (Collection)This collection includes information about promoters, operators, CDSs and proteins from Bacillus subtilis. Functional interactions such as transcriptional activation and repression, protein production and various protein-protein interactions are also included.
BBa_J24823BBa_J24823 Version 1 (Component)same as J24819 but with ACCACC Euk RBS removed and problem solved via J24822 removed
BBa_K079021BBa_K079021 Version 1 (Component)LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.