BBa_K750004BBa_K750004 Version 1 (Component)LuxI expression device activated by arabinose(Regulated by RBS of 0.07 strength)
BBa_K750005BBa_K750005 Version 1 (Component)LuxI expression device activated by arabinose(Regulated by RBS of 0.01 strength)
BBa_K750006BBa_K750006 Version 1 (Component)LuxR expression device activated by arabinose
BBa_K737058BBa_K737058 Version 1 (Component)Spot42 is a multitarget small RNA that mediates the discoordinate expression of the E.coli galactose
BBa_K750008BBa_K750008 Version 1 (Component)Quorum sensing system based on LuxI and LuxR to control the expression of parts behind
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
BBa_K098992BBa_K098992 Version 1 (Component)mtrB, high expression
BBa_K098990BBa_K098990 Version 1 (Component)mtrB, low expression
BBa_K098984BBa_K098984 Version 1 (Component)lac inducible mtrB (high expression of QPI)
BBa_K098983BBa_K098983 Version 1 (Component)lac inducible mtrB (low expression of QPI)
BBa_K098010BBa_K098010 Version 1 (Component)HO-pcyA, low expression
BBa_K143077BBa_K143077 Version 1 (Component)P43-gsiB GFP expression construct
BBa_K143078BBa_K143078 Version 1 (Component)P43-spoVG GFP expression construct
BBa_K143079BBa_K143079 Version 1 (Component)Pveg-spoVG GFP expression construct
BBa_K143080BBa_K143080 Version 1 (Component)P43-gsiB RFP expression construct
BBa_K143081BBa_K143081 Version 1 (Component)P43-spoVG RFP expression construct
BBa_K143082BBa_K143082 Version 1 (Component)Pveg-spoVG RFP expression construct
pBSEP1BBa_K090401 Version 1 (Component)Gram-positive Shuttle Vector for Episomal Expression
BBa_K142047BBa_K142047 Version 1 (Component)tet-controlled LacI generator with constitutive TetR expression cassette
BBa_K142024BBa_K142024 Version 1 (Component)IPTG-on tetracycline-off pulse generator with LacI mutant (R197A) and TetR expression cassette
BBa_K142025BBa_K142025 Version 1 (Component)IPTG-on tetracycline-off pulse generator with LacI mutant (R197F) and TetR expression cassette
BBa_K142026BBa_K142026 Version 1 (Component)IPTG-on tetracycline-off pulse generator with LacI mutant (T276A) and TetR expression cassette
BBa_K142027BBa_K142027 Version 1 (Component)IPTG-on tetracycline-off pulse generator with LacI mutant (T276F) and TetR expression cassette
BBa_K142028BBa_K142028 Version 1 (Component)IPTG-on tetracycline-off pulse generator with LacI mutant (R197A, T276A)/TetR expression cassette
BBa_K142029BBa_K142029 Version 1 (Component)IPTG-on tetracycline-off pulse generator with LacI mutant (R197A, T276F)/TetR expression cassette
BBa_K142030BBa_K142030 Version 1 (Component)IPTG-on tetracycline-off pulse generator with LacI mutant (R197F, T276A)/TetR expression cassette
BBa_K142031BBa_K142031 Version 1 (Component)IPTG-on tetracycline-off pulse generator with LacI mutant (R197F, T276F)/TetR expression cassette
BBa_K142073BBa_K142073 Version 1 (Component)lac-controlled GFP generator with constitutive lacI expression cassette
BBa_K142074BBa_K142074 Version 1 (Component)lac-controlled GFP-LVA generator with constitutive lacI expression cassette
BBa_K142075BBa_K142075 Version 1 (Component)lac-controlled CFP generator with constitutive lacI expression cassette
BBa_K142076BBa_K142076 Version 1 (Component)lac-controlled RFP generator with constitutive lacI expression cassette
BBa_K142048BBa_K142048 Version 1 (Component)promotorless lacI expression cassette followed by constitutively active TetR generator
T7pBBa_K133041 Version 1 (Component)T7 promoter expression plasmid backbone
trfABBa_K112131 Version 1 (Component)TrfA constitutive expression cassette
BBa_K112133BBa_K112133 Version 1 (Component)Xis Int temperature sensitive expression cassette
BBa_K112137BBa_K112137 Version 1 (Component)Xis Int & TrfA combined expression cassette
BBa_J70121BBa_J70121 Version 1 (Component)R0040 regulated expression of a lacZa.GFP fusion protein
BBa_J70029BBa_J70029 Version 1 (Component)RBS + mCherry gene, optimized for expression in Me. florum and E. coli
BBa_P111102BBa_P111102 Version 1 (Component)BAMT Expression Cassette (R0040)
BBa_P121102BBa_P121102 Version 1 (Component)BAMT Expression Cassette (F2620)
BBa_P111103BBa_P111103 Version 1 (Component)JMT Expression Cassette (R0040,PCR)
BBa_P121103BBa_P121103 Version 1 (Component)JMT Expression Cassette (F2620, PCR)
BBa_P111104BBa_P111104 Version 1 (Component)JMT Expression Cassette (R0040, Synth)
BBa_P121104BBa_P121104 Version 1 (Component)JMT Expression Cassette (F2620,Synth)
BBa_P111105BBa_P111105 Version 1 (Component)SAAT Expression Cassette (R0040)
BBa_P121105BBa_P121105 Version 1 (Component)SAAT Expression Cassette (F2620)
BBa_P111106BBa_P111106 Version 1 (Component)BEAT Expression Cassette (R0040)
BBa_P121106BBa_P121106 Version 1 (Component)BEAT Expression Cassette (F2620)
BBa_P111107BBa_P111107 Version 1 (Component)EHT1 Expression Cassette (R0040)