Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chris Hirst
Date created: 2008-10-27 12:00:00
Date modified: 2015-05-08 01:10:24
P43-gsiB RFP expression construct
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K143080_sequence (Version 1) |
Description
RFP gene under expresson of the P43 promoter and gsiB RBS of B. subtilis, with a chloramphenicol resistance marker for ease of selection.Also contains the 5' and 3' amyE integration sequences to allow integration into B. subtilis at the amyE locus.
This device was used by the Imperial iGEM 2008 team to characterise the P43 promoter and gsiB RBS (Combined part
Notes
The 5' and 3' amyE integration sequences were PCR cloned from the B. subtilis integration vector pDR111 using Pfu DNA polymerase. The double terminator, RFP gene and chlorapmphenicol acetyltransferase gene were taken from the registry. The sequence of promoter P43 and RBS gsiB were taken from papers.Source
The 5' and 3' amyE integration sequences were PCR cloned from the B. subtilis integration vector pDR111 utilising Pfu DNA polymerase and cloned into a BioBrick with the parts from the registry and the P43-gsiB part that was synthesised by GeneArt.| Access | Instance | Definition |
| public public | BBa_K143071 BBa_K143076 | BBa_K143071 BBa_K143076 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K143071 BBa_K143076 | 1,1491 1498,3350 | BBa_K143071 BBa_K143076 |