Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chris Hirst
Date created: 2008-10-27 12:00:00
Date modified: 2015-05-08 01:10:24
AmyE integratable PoPS generator (P43-gsiB) (with CmR)
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K143071_sequence (Version 1) |
Description
AmyE 5' Integration sequence(The amyE 5' integration sequence allows integration into the B. subtilis genome at the amyE locus if the 3' amyE integration sequence(
The chloramphenicol adenyltransferase give resistance to chloramphenicol while the terminator prevents readthrough.
The P43-gsiB promoter and RBS for B. subtilis constitutively generate a PoPS output.
Notes
The amyE 5' integration sequence was PCR cloned from the B. subtilis integration vector pDR111 utilising Pfu DNA polymerase. The sequence of P43-gsiB was obtained from papers. Chloramphenicol adenyltransferase and the double terminator were obtained from the registry.Source
The amyE 5' integration sequence was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick with the P43-gsiB that was synthesised by GeneArt and Chloramphenicol adenyltransferase and the double terminator that were obtained from the registry.| Access | Instance | Definition |
| public public public public public public public public | BBa_K143001 BBa_K143013 BBa_K143020 BBa_J31005 BBa_B0010 BBa_B0012 BBa_K143013 BBa_K143020 | amyE 5 IS P43 RBS-GsiB CmR BBa_B0010 BBa_B0012 P43 RBS-GsiB |