BBa_S01430
BBa_S01430 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01427
BBa_S01427 Version 1 (Component)
Intermediate part from assembly 240

SspDnaB(C)
BBa_K1362409 Version 1 (Component)
SspDnaB C-Intein cloning piece

BBa_S01428
BBa_S01428 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01431
BBa_S01431 Version 1 (Component)
Intermediate part from assembly 240

BBa_K1453101
BBa_K1453101 Version 1 (Component)
[ssDsbA]->[RFP]->[Lgt]->[TAL-adapter]

1Gate-NpuD
BBa_K1362100 Version 1 (Component)
RBS + NpuDnaE N-intein RFC[105] assembly construct (with His6)

BBa_M36983
BBa_M36983 Version 1 (Component)
Type II Secreted Antifreeze Actuator in E. coli (5-Repeat) Correct

BBa_M36982
BBa_M36982 Version 1 (Component)
Type II Secreted Antifreeze Actuator in E. coli (1-Repeat) Correct

BBa_I5510
BBa_I5510 Version 1 (Component)
Olmecs: Synchro-Dualator part 2

BBa_K195408
BBa_K195408 Version 1 (Component)
This part is composed of PelB,F10scFv and C-IgAP

BBa_K2170140
BBa_K2170140 Version 1 (Component)
N-terminal part of prokaryotic biotin binding receptor (TetR_TetO_OmpA_eMA) in RFC[25] N-part

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_S03254
BBa_S03254 Version 1 (Component)
Intermediate part from assembly 317

BBa_S03247
BBa_S03247 Version 1 (Component)
Intermediate part from assembly 317

BBa_S03260
BBa_S03260 Version 1 (Component)
Intermediate part from assembly 317

BBa_S03255
BBa_S03255 Version 1 (Component)
Intermediate part from assembly 317

BBa_S01924
BBa_S01924 Version 1 (Component)
Intermediate part from assembly 283

BBa_S03171
BBa_S03171 Version 1 (Component)
Intermediate part from assembly 317

BBa_S03252
BBa_S03252 Version 1 (Component)
Intermediate part from assembly 317

BBa_S03250
BBa_S03250 Version 1 (Component)
Intermediate part from assembly 317

BBa_S01926
BBa_S01926 Version 1 (Component)
Intermediate part from assembly 283

BBa_S03264
BBa_S03264 Version 1 (Component)
Intermediate part from assembly 317

BBa_S03257
BBa_S03257 Version 1 (Component)
Intermediate part from assembly 317

BBa_S03261
BBa_S03261 Version 1 (Component)
Intermediate part from assembly 317

BBa_K1159304
BBa_K1159304 Version 1 (Component)
Signal Peptide of Ig Kappa chain from <i>Mus musculus</i> in RFC[25] N-part

1GateNpuE
BBa_K1362101 Version 1 (Component)
RBS+NpuDnaE C-intein RFC[105] assembly construct (with His6)

BBa_S01291
BBa_S01291 Version 1 (Component)
Intermediate part from assembly 236

BBa_S01639
BBa_S01639 Version 1 (Component)
Intermediate part from assembly 240

BBa_K307106
BBa_K307106 Version 1 (Component)
parB

BBa_K307101
BBa_K307101 Version 1 (Component)
parS

BBa_K568003
BBa_K568003 Version 1 (Component)
T7 promoter lacZ reporter part

BBa_J07033
BBa_J07033 Version 1 (Component)
Composite part for FecA' (promoter::rbs::FecA')

BSMT2
BBa_J45007 Version 1 (Component)
SAM:benzoic acid/salicylic acid carboxyl methyltransferase II; converts salicylic acid to methyl sal

BBa_K106692
BBa_K106692 Version 1 (Component)
Sir3, AarI A!D part (A!D variant!)

BBa_K1051974
BBa_K1051974 Version 1 (Component)
clb6 P + D-box + GFP + MER2 Intron + TOM40 + T

BBa_S03490
BBa_S03490 Version 1 (Component)
--Specify Parts List--

BBa_K223020
BBa_K223020 Version 1 (Component)
WCP + RBS + SoxR + TT + SoxS + RBS + BLH + RBS + RALDH II + TT

BBa_K223019
BBa_K223019 Version 1 (Component)
MCP + RBS + SoxR + TT + SoxS + RBS + BLH + RBS + RALDH II + TT

BBa_K223018
BBa_K223018 Version 1 (Component)
SCP + RBS + SoxR + TT + SoxS + RBS + BLH + RBS + RALDH II + TT

BBa_S01925
BBa_S01925 Version 1 (Component)
Intermediate part from assembly 283

BBa_S03262
BBa_S03262 Version 1 (Component)
Intermediate part from assembly 317

BBa_S03248
BBa_S03248 Version 1 (Component)
Intermediate part from assembly 317

BBa_S03131
BBa_S03131 Version 1 (Component)
Intermediate part from assembly 299

BBa_S03258
BBa_S03258 Version 1 (Component)
Intermediate part from assembly 317

BBa_S03256
BBa_S03256 Version 1 (Component)
Intermediate part from assembly 317

BBa_S01276
BBa_S01276 Version 1 (Component)
Intermediate part from assembly 236

BBa_S01290
BBa_S01290 Version 1 (Component)
Intermediate part from assembly 236

BBa_S01289
BBa_S01289 Version 1 (Component)
Intermediate part from assembly 236

BBa_S01477
BBa_S01477 Version 1 (Component)
Intermediate part from assembly 233