BBa_K1956024BBa_K1956024 Version 1 (Component)Noise in Device measurement
BBa_K1956027BBa_K1956027 Version 1 (Component)Noise in Device measurement
BBa_K2123202BBa_K2123202 Version 1 (Component)MerT + MerP (Hg transporters) + MerB (Organomercurial Lyase) + MerA (Mercuric Reductase) + B0015
BBa_K091135BBa_K091135 Version 1 (Component)Las Sender Testing Device
BBa_K239013BBa_K239013 Version 1 (Component)tetR consititutive YFP Device
BBa_I3510BBa_I3510 Version 1 (Component)Quorum receiver device (R0063.I0462.R0062)
BBa_K886002BBa_K886002 Version 1 (Component)Recombination Device composed by Cre-lox71
BBa_K594011BBa_K594011 Version 1 (Component)A device that can accepts the 3--O-C6-HSL and then produces 3-O-C12-HSL and ECFP reporter.
BBa_K594014BBa_K594014 Version 1 (Component)A device that can accepts the 3--OH-C14:1-HSL and then produces 3-O-C6-HSL and GFP reporter.
BBa_K317033BBa_K317033 Version 1 (Component)3OC6HSL Regurated T4 Lysis device
BBa_K317032BBa_K317032 Version 1 (Component)IPTG regulated T4 Lysis device
BBa_K1954007BBa_K1954007 Version 1 (Component)Green Light Inducible bacteriocin Device (GLID)
BBa_K779603BBa_K779603 Version 1 (Component)Hef1a-eYFP-4xFF1 MammoBlock Device
BBa_J92001BBa_J92001 Version 1 (Component)Lead Remover and Reporter Device
pTetR-LacIBBa_I763030 Version 1 (Component)LacI coding device regulated by pTetR
BBa_K299029BBa_K299029 Version 1 (Component)RBS measurement device pT7 J61127 GFP
BBa_I763003BBa_I763003 Version 1 (Component)GFP coding device switched on by IPTG
BBa_K2123110BBa_K2123110 Version 1 (Component)Improved Mer Operon Device with novel regulated exponential promoter
BBa_K1606010BBa_K1606010 Version 1 (Component)Kumamax enzyme that gluten updated m-cherry reporter device
BBa_K511905BBa_K511905 Version 1 (Component)Repressible rtTA3 Transactivator Generator (Hef1a-LacO-rtTA3) MammoBlock Device
BBa_K1341011BBa_K1341011 Version 1 (Component)OR LOGIC GATE IN Graph Theory (GFP OUTPUT DEVICE)
BBa_K137033BBa_K137033 Version 1 (Component)Device with GFP with (AC)21 repeat after start codon
pCMV-ECFP-BBa_I763023 Version 1 (Component)LacI coding device with ECFP as a reporter regulated by pCMV
BBa_J04431BBa_J04431 Version 1 (Component)GFP Coding Device with promoter, RBS, GFP with LVA tag, and Terminator
Adapter BiBBa_K1807015 Version 1 (Component)This device allows for the IPTG-inducible expression of lacZα peptide which in the presence of
iGEM Parts Registryigem_collection Version 1 (Collection)The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.