BBa_J107039BBa_J107039 Version 1 (Component)heat-inducible device + NOT gate + RFP
BBa_K1676075BBa_K1676075 Version 1 (Component)GFP Reporter with Mutant RBS Opt
BBa_J58003BBa_J58003 Version 1 (Component)This is not a part. This is a bomb.
BBa_K411000BBa_K411000 Version 1 (Component)Testing registry entry. This part doeth not exist.
BBa_K1077006BBa_K1077006 Version 1 (Component)nat fim switch b0034 GFP OFF orientation
BBa_J70585BBa_J70585 Version 1 (Component)Lpp promoter (sequence only-- not shifted relative to scar)
BBa_M36556BBa_M36556 Version 1 (Component)5' Bicistronic UTR (medium), does not include ATG start
HaxBBa_K116999 Version 1 (Component)Testing registry entry. This part doeth not exist.
BBa_J70586BBa_J70586 Version 1 (Component)Mutant Glns promoter (sequence only not shifted relative to scar)
BBa_K1524106BBa_K1524106 Version 1 (Component)stemmed anti-sense mRFP (150 nt)
BBa_K1524104BBa_K1524104 Version 1 (Component)stemmed anti-sense mRFP (90 nt)
BBa_K1524105BBa_K1524105 Version 1 (Component)stemmed anti-sense mRFP (120 nt)
BBa_K1649002BBa_K1649002 Version 1 (Component)Red light sensor with TetR not gate and GFP reporter
BBa_K222003BBa_K222003 Version 1 (Component)Mid1 gene disrupted from 489 to 2021 nt
BBa_K222004BBa_K222004 Version 1 (Component)Cch1 gene disrupted from 532 to 6708 nt
LaLaLaBBa_K1479001 Version 1 (Component)0.0 Testing the UE of the bio-brick upload page, not designing a real one
BBa_K222002BBa_K222002 Version 1 (Component)project of a Mid1 gene disrupted from 489 to 2021 nt
BBa_M36305BBa_M36305 Version 1 (Component)Transcription terminator pT-T7 from bacteriophage
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.