BBa_K1173502
BBa_K1173502 Version 1 (Component)
<i>ompC</i> w/GFP + (<i>BglII+BamHI</i>)restriction sites

BBa_K1045015
BBa_K1045015 Version 1 (Component)
RBS BBa_B0034 with inversed Pre- and Suffix- Promoter reverse - Promoter - DarR operator - BBa_E0240

BBa_K346029
BBa_K346029 Version 1 (Component)
T7p(BBa_I719005)+RBS(B0034)+Lpp-OmpA+MBP(surface display of lead binding peptide)

BBa_K1694053
BBa_K1694053 Version 1 (Component)
Pcons+B0034+Lpp-OmpA-N+scFv(Anti-VEGF)+B0034+amilCP+B0015

BBa_K1694035
BBa_K1694035 Version 1 (Component)
Pcons+B0034+Lpp-OmpA-N+scFv(Anti-HER2)+B0030+GFP+J61048

BBa_K195403
BBa_K195403 Version 1 (Component)
This part is composed of pTetR,RBS,Lpp-OmpA-linker and F10scFv

BBa_K336061
BBa_K336061 Version 1 (Component)
promoter+RBS+ompA signal+lacZ+double termnator+promoter+RBS+SBFP2+double termnator

BBa_K1124123
BBa_K1124123 Version 1 (Component)
inverse PCR template for creating new sRNA (plambda-micC sRNA scaffold-terminator)

BBa_K127003
BBa_K127003 Version 1 (Component)
Polystyrene binding peptide inserted CPX generated by qurum sensing and constitutive GFP genarator

BBa_K195404
BBa_K195404 Version 1 (Component)
This part is composed of pTetR,RBS,Lpp-OmpA-linker,F10scFv and terminator

BBa_K769003
BBa_K769003 Version 1 (Component)
Pconst.(High)-RBS-NpSRII-9a.a.linker_NpHtrII-EnvZ-Double terminator-PompR-RBS-GFP-Double terminator

BBa_K542008
BBa_K542008 Version 1 (Component)
pLacI Regulated Lumazine Synthase and pBAD Inverse-Regulated Arg-tagged ECFP and EYFP

BBa_J36849
BBa_J36849 Version 1 (Component)
Lac-inducible generator of Lpp-OmpA(46-66)-Streptavidin single-chain dimeric + His6 tag

BBa_J36851
BBa_J36851 Version 1 (Component)
Lac-inducible generator of Lpp-OmpA(46-159)-Streptavidin single-chain dimeric + His6 tag

BBa_K1520516
BBa_K1520516 Version 1 (Component)
MC31-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter

BBa_K1520515
BBa_K1520515 Version 1 (Component)
MC7-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter.

BBa_K341555
BBa_K341555 Version 1 (Component)
CBD+OmpA

SS_ompA
BBa_K257006 Version 1 (Component)
ompA sequence signal

BBa_K346006
BBa_K346006 Version 1 (Component)
Dsba-mbp(lead metal binding peptide)+mbp(lead metal binding peptide)+lpp-ompa-mbp(lead metal binding

BBa_K1520510
BBa_K1520510 Version 1 (Component)
PgolTS-golS-PgolB-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter

BBa_K1132014
BBa_K1132014 Version 1 (Component)
Promoter (OmpR, positive) followed by RFP protein generator

BBa_M11085
BBa_M11085 Version 1 (Component)
E coli outer membrane protein C (ompC) with BamHI RE site for insertion of gene to be expressed on o

BBa_K660605
BBa_K660605 Version 1 (Component)
OmpF + wYFP + TT

BBa_J58014
BBa_J58014 Version 1 (Component)
Promoter activated by OmpR-P with the reporter GFP

BBa_K310004
BBa_K310004 Version 1 (Component)
ompF 5'UTR (micF target)

BBa_K2036028
BBa_K2036028 Version 1 (Component)
placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-ompA-iLDH-TT-pRM-RBS-beta-galactosidase

BBa_K877001
BBa_K877001 Version 1 (Component)
LPP-OmpA and CtCoh2 Combination

BBa_K1909013
BBa_K1909013 Version 1 (Component)
Omp Promoter / GFP

BBa_J58015
BBa_J58015 Version 1 (Component)
Mutated promoter activated by OmpR-P with the reporter GFP

BBa_K987001
BBa_K987001 Version 1 (Component)
This is a composite part which has the function to invert the temperature activation by the part: BB

BBa_K387007
BBa_K387007 Version 1 (Component)
OmpA: An excretory signal peptide in bacteria

BBa_K212002
BBa_K212002 Version 1 (Component)
OmpC-RFP (promoter-reporter transduction readout)

BBa_K195202
BBa_K195202 Version 1 (Component)
Lpp-OmpA-linker+alpha-V integrin gene+terminator

BBa_K195203
BBa_K195203 Version 1 (Component)
RBS+Lpp-OmpA-linker+alpha-V integrin gene+terminator

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.