Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Perry Tsai
Date created: 2006-10-28 11:00:00
Date modified: 2015-08-31 04:08:47
Lac-inducible generator of Lpp-OmpA(46-159)-Streptavidin single-chain dimeric + His6 tag
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_J36851_sequence (Version 1) |
Description
This part codes for the core part of wild-type streptavidin protein, without the start codon. Streptavidin protein binds strongly to the biotin molecule, and can be used to bind biotinylated nucleic acids or peptides. However, this version has been mutated so that it will not bind biotin strongly.It was used as part of a fusion protein with lipoprotein signal peptide and outer membrane protein A transmembrane domains, to express streptavidin on the outer surface of E. coli. This dead mutant would serve as a type of negative control.
Notes
There was originally a XbaI site TCTAGA at nucleotides 118-123 of the part, which was mutated to AGCCGC. It is missing its start codon at the beginning, as the intention was to fuse this with surface display domains upstream.There are no spacer nucleotides between the part and the XbaI restriction site, or between the part and the SpeI restriction site, so that the mixed site would be 6bp long, and reading frame would be maintained between protein domains.
Source
PCR off a plasmid obtained from Alice Ting's lab at MIT.| Access | Instance | Definition |
| public public public public public | BBa_R0010 BBa_B0034 BBa_J36835 BBa_J36837 BBa_J36843 | LacI BBa_B0034 BBa_J36835 BBa_J36837 BBa_J36843 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0010 BBa_B0034 BBa_J36835 BBa_J36837 BBa_J36843 | 1,200 209,220 227,313 322,663 672,1490 | LacI BBa_B0034 BBa_J36835 BBa_J36837 BBa_J36843 |