BBa_K777105
BBa_K777105 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23109

BBa_K777106
BBa_K777106 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23112

BBa_K777107
BBa_K777107 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23113

BBa_K777108
BBa_K777108 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23114

BBa_K777125
BBa_K777125 Version 1 (Component)
Constitutive J23100 promoter

BBa_K098994
BBa_K098994 Version 1 (Component)
mtrB with RBS and terminator

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

BBa_K098993
BBa_K098993 Version 1 (Component)
heat sensitive cI QPI with low promoter

BBa_K098990
BBa_K098990 Version 1 (Component)
mtrB, low expression

BBa_K098987
BBa_K098987 Version 1 (Component)
temperature sensitive cI inducible system with GFP reporter and low promoter

BBa_K098985
BBa_K098985 Version 1 (Component)
lac QPI driven by low promoter

BBa_K098982
BBa_K098982 Version 1 (Component)
lac inducible system with low promoter and GFP reporter

BBa_K098983
BBa_K098983 Version 1 (Component)
lac inducible mtrB (low expression of QPI)

BBa_K098980
BBa_K098980 Version 1 (Component)
tet QPI driven by low promoter

BBa_K098010
BBa_K098010 Version 1 (Component)
HO-pcyA, low expression

BBa_K098011
BBa_K098011 Version 1 (Component)
E. coli ompR, expressed at low level

BBa_K086001
BBa_K086001 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter

BBa_K086018
BBa_K086018 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24

BBa_K086002
BBa_K086002 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP

BBa_K086019
BBa_K086019 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24

BBa_K086003
BBa_K086003 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter

BBa_K086020
BBa_K086020 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24

BBa_K090503
BBa_K090503 Version 1 (Component)
Gram-Positive General Constitutive Promoter

BBa_K090504
BBa_K090504 Version 1 (Component)
Gram-Positive Strong Constitutive Promoter

BBa_K142008
BBa_K142008 Version 1 (Component)
lacI IS mutant R197A with RBS and terminator

BBa_K142009
BBa_K142009 Version 1 (Component)
lacI IS mutant R197F with RBS and terminator

BBa_K142010
BBa_K142010 Version 1 (Component)
lacI IS mutant T276A with RBS and terminator

BBa_K142011
BBa_K142011 Version 1 (Component)
lacI IS mutant T276Fwith RBS and terminator

BBa_K142012
BBa_K142012 Version 1 (Component)
lacI IS mutant R197A T276A with RBS and terminator

BBa_K142013
BBa_K142013 Version 1 (Component)
lacI IS mutant R197A T276F with RBS and terminator

BBa_K142014
BBa_K142014 Version 1 (Component)
lacI IS mutant R197F T276A with RBS and terminator

BBa_K142015
BBa_K142015 Version 1 (Component)
lacI IS mutant R197F T276F with RBS and terminator

BBa_K156021
BBa_K156021 Version 1 (Component)
Promoter + RBS + phaA + double terminator

BBa_K156022
BBa_K156022 Version 1 (Component)
Promoter + RBS + phaB1 + double terminator

BBa_K156023
BBa_K156023 Version 1 (Component)
Promoter + RBS + phaC1 + double terminator

BBa_K142202
BBa_K142202 Version 1 (Component)
SceI with RBS (BBa_B0030) and double terminator (BBa_B0015)

BBa_K142203
BBa_K142203 Version 1 (Component)
T4 ligase with RBS (BBa_B0030) and double terminator (BBa_B0015)

BBa_K142204
BBa_K142204 Version 1 (Component)
lac Promoter + RBS + T4 ligase + double terminator

BBa_K142205
BBa_K142205 Version 1 (Component)
Plac + RBS + SceI + double terminator

BBa_K106679
BBa_K106679 Version 1 (Component)
GFP, under pheromone-inducible promoter, 5' LexA Ops

BBa_K106674
BBa_K106674 Version 1 (Component)
GFP under medium constitutive promoter, 5

BBa_K106675
BBa_K106675 Version 1 (Component)
mCherry under medium constitutive promoter

BBa_K142206
BBa_K142206 Version 1 (Component)
strong constitutive T4 ligase generator

BBa_K142207
BBa_K142207 Version 1 (Component)
medium constitutive T4 ligase generator

BBa_K106694
BBa_K106694 Version 1 (Component)
Adh1P! (Adh1 Promoter, A! end)

BBa_K142047
BBa_K142047 Version 1 (Component)
tet-controlled LacI generator with constitutive TetR expression cassette

Sir3
BBa_K106695 Version 1 (Component)
Sir3 under a strong constitutive promoter

BBa_K116001
BBa_K116001 Version 1 (Component)
nhaA promoter, that can be regulated by pH and nhaR protein.

BBa_K086021
BBa_K086021 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24