BBa_K137065
BBa_K137065 Version 1 (Component)
Promoter with (C)10 repeat in front of RBS-LacZ-TT

BBa_K137063
BBa_K137063 Version 1 (Component)
Promoter with (TA)10 repeat in front of RBS-LacZ-TT

BBa_K323075
BBa_K323075 Version 1 (Component)
ATG cYFP link HIVC

BBa_K1088053
BBa_K1088053 Version 1 (Component)
GFP reporter with flexible linker at N-terminus for creation of GFP fusions

BBa_J24822
BBa_J24822 Version 1 (Component)
Same as J24819 but with the error at the luc-terminator junction fixed

BBa_I715046
BBa_I715046 Version 1 (Component)
T7pro-RBS-RFP1-HixC-RFP2-RBS-GFP1-HixC-GFP2-TT-HixC

BBa_K855006
BBa_K855006 Version 1 (Component)
pvdQ gene with a silent mutation at 1494 bp to remove the internal PstI site

BBa_K855005
BBa_K855005 Version 1 (Component)
pvdQ gene with a silent mutation at 1491 bp to remove the internal PstI site

BBa_K223033
BBa_K223033 Version 1 (Component)
trp-operon + RBS + IL-6 + HlyA + TT Trp-sensitive IL-6 synthesizer

BBa_J119408
BBa_J119408 Version 1 (Component)
Pupp promoter mutant - Substitution of C and G to A at 28 and 30

BBa_K202004
BBa_K202004 Version 1 (Component)
Hybrid promoter having multiple operator sites. Promoter has tetO2 with mutation at position 3

BBa_K199194
BBa_K199194 Version 1 (Component)
TT-pLux-RBS-LuxI 2 sites-RBS-GFP-RBS-RFP-pLacI-RBS-LuxR

BBa_K1022125
BBa_K1022125 Version 1 (Component)
pT7: RBS: His6- SUMO: Signiferin: RBS: TetR:TT : pTet: cI: TT : pcI: Ulp : Lysis

BBa_K199193
BBa_K199193 Version 1 (Component)
TT-pLux-RBS-LuxI 2 sites-RBS-RFP-RBS-GFP-pBAD-RBS-LuxR

BBa_M36427
BBa_M36427 Version 1 (Component)
Recombinase Site 1 (Bxb1 AttB)

BBa_K1088059
BBa_K1088059 Version 1 (Component)
GFP reporter with flexible linker at N-terminus for creation of GFP fusions

BBa_K1088052
BBa_K1088052 Version 1 (Component)
GFP reporter with flexible linker at N-terminus for creation of GFP fusions

BBa_J100017
BBa_J100017 Version 1 (Component)
TT+pLux+RBS+LuxI(2-SAT 2 clause)+RBS+GFP+pLac+RBS+LuxR+tRNAs

BBa_K1022126
BBa_K1022126 Version 1 (Component)
pT7: RBS: His6- SUMO: Magainin II: RBS: TetR:TT : pTet: cI: TT : pcI: Ulp : Lysis

BBa_K1361005
BBa_K1361005 Version 1 (Component)
CsgE, CsgF, CsgG, the outer membrane secrete device for curli fiber, at relatively low constitutive

BBa_J107021
BBa_J107021 Version 1 (Component)
aTc sensor (J23106 promoter) with GFP

BBa_K820014
BBa_K820014 Version 1 (Component)
mazF-TT

BBa_J31016
BBa_J31016 Version 1 (Component)
part produces the RNA construct crRNA-RBS-GFPLVA-tt that can only be translated in the presence of t

BBa_K199112
BBa_K199112 Version 1 (Component)
RFP-TT intermediate

BBa_K660605
BBa_K660605 Version 1 (Component)
OmpF + wYFP + TT

BBa_M36556
BBa_M36556 Version 1 (Component)
5' Bicistronic UTR (medium), does not include ATG start

BBa_K1361007
BBa_K1361007 Version 1 (Component)
Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag at a relative

BBa_K318501
BBa_K318501 Version 1 (Component)
lacI pL + RBS + RcsB + TT

BBa_K199165
BBa_K199165 Version 1 (Component)
pT7 + RBS + GFP + TT in pSB3T5

BBa_S03737
BBa_S03737 Version 1 (Component)
pLac-lox-RFP(reverse)-TT-lox-RBS-Tet (psB1A2)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.