BBa_K2092002
BBa_K2092002 Version 1 (Component)
PalcA, improved alcR inducible promoter from A. nidulans

BBa_K590046
BBa_K590046 Version 1 (Component)
AAR-PSB3K3-Lac Inducible w/o LacI

BBa_I3400
BBa_I3400 Version 1 (Component)
Hk022cI switch input device (B0034.C0050.B0015)

PchiP-lacZ
BBa_K564002 Version 1 (Component)
Chitoporin fused with lacZ - target for sRNA based regulation

BBa_I763003
BBa_I763003 Version 1 (Component)
GFP coding device switched on by IPTG

BBa_K638201
BBa_K638201 Version 1 (Component)
Arabinose inducible Poly-His Reflectin A1 generator

BBa_K1088010
BBa_K1088010 Version 1 (Component)
E. coli dxs-GFP protein fusion (lac promoter with LVA-tagged lac inhibitor (LacI:LVA) - IPTG inducib

BBa_K418001
BBa_K418001 Version 1 (Component)
From partsregistry.org IPTG inducible Lac promoter cassette

Prom/RBS
BBa_K262000 Version 1 (Component)
BBa_R0011 & BBa_B0034, IPTG-inducible promoter with Elowitz RBS.

BBa_R4030
BBa_R4030 Version 1 (Component)
PoPS/RiPS Generator composed of the Tet promoter and a strong RBS (R0040.E0030)

BBa_I3401
BBa_I3401 Version 1 (Component)
Lambda cI switch input device (B0034.C0051.B0015)

BBa_K2123110
BBa_K2123110 Version 1 (Component)
Improved Mer Operon Device with novel regulated exponential promoter

BBa_K1088057
BBa_K1088057 Version 1 (Component)
T25 domain of bacterial two-hybrid system (IPTG inducible)

BBa_K1606010
BBa_K1606010 Version 1 (Component)
Kumamax enzyme that gluten updated m-cherry reporter device

BBa_K511905
BBa_K511905 Version 1 (Component)
Repressible rtTA3 Transactivator Generator (Hef1a-LacO-rtTA3) MammoBlock Device

BBa_K1341011
BBa_K1341011 Version 1 (Component)
OR LOGIC GATE IN Graph Theory (GFP OUTPUT DEVICE)

BBa_K1968009
BBa_K1968009 Version 1 (Component)
PglaA inducible promoter Phytobrick: glucoamylase gene promoter (PglaA) from Aspergillus niger

AraC_TEV-F
BBa_K627008 Version 1 (Component)
Fusion part of arabinose-inducible induction system and the TEV protease

BBa_K563053
BBa_K563053 Version 1 (Component)
vector pYE, designed for inducible expression of recombinant proteins in S.cerevisivae.

Adapter Bi
BBa_K1807000 Version 1 (Component)
Protein generator device suitable for blue-white screening and Gibson Assembly.

BBa_K137033
BBa_K137033 Version 1 (Component)
Device with GFP with (AC)21 repeat after start codon

pSBBs0K
BBa_K823026 Version 1 (Component)
pSB_Bs0K-P_spac (replicative Bacillus subtilis expression vector; IPTG inducible

pCMV-ECFP-
BBa_I763023 Version 1 (Component)
LacI coding device with ECFP as a reporter regulated by pCMV

BBa_J04431
BBa_J04431 Version 1 (Component)
GFP Coding Device with promoter, RBS, GFP with LVA tag, and Terminator

BBa_K1075003
BBa_K1075003 Version 1 (Component)
Promoter(const.)-AraC-Term-pBAD-RBS34 (Arabinose inducable promoter system)

BBa_K2123117
BBa_K2123117 Version 1 (Component)
Novel RFP device regulated by mercury: MerR (regulatory protein) + Stationary phase with mer operato

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

iGEM Parts Registry
igem_collection Version 1 (Collection)
The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.