BBa_S01297
BBa_S01297 Version 1 (Component)
Intermediate part from assembly 236

BBa_S01298
BBa_S01298 Version 1 (Component)
Intermediate part from assembly 236

BBa_S01299
BBa_S01299 Version 1 (Component)
Intermediate part from assembly 236

BBa_K821002
BBa_K821002 Version 1 (Component)
Orthogonal 16S DNA sequence from rrnB

BBa_K821003
BBa_K821003 Version 1 (Component)
16S DNA sequence from rrnB

BBa_K821005
BBa_K821005 Version 1 (Component)
CmR label from pKD3

BBa_K821006
BBa_K821006 Version 1 (Component)
KanR label from pKD3

BBa_S01300
BBa_S01300 Version 1 (Component)
Intermediate part from assembly 236

BBa_J72159
BBa_J72159 Version 1 (Component)
rbs.repA from ColE2-P9 plasmid

BBa_J72160
BBa_J72160 Version 1 (Component)
rbs2.repA from ColE2-P9 plasmid

BBa_J72164
BBa_J72164 Version 1 (Component)
dxs ORF from E. coli MG1655

BBa_S01301
BBa_S01301 Version 1 (Component)
Intermediate part from assembly 236

BBa_S01302
BBa_S01302 Version 1 (Component)
Intermediate part from assembly 236

BBa_S01303
BBa_S01303 Version 1 (Component)
Intermediate part from assembly 236

BBa_K737000
BBa_K737000 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

BBa_K737001
BBa_K737001 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

BBa_S01304
BBa_S01304 Version 1 (Component)
Intermediate part from assembly 236

BBa_K817019
BBa_K817019 Version 1 (Component)
parAB operon from pseudomonas putida KT2440 chromosome

BBa_K863006
BBa_K863006 Version 1 (Component)
ecol laccase from E. coli

BBa_K863005
BBa_K863005 Version 1 (Component)
ecol laccase from E. coli with T7 promoter, RBS and His-tag

BBa_K863000
BBa_K863000 Version 1 (Component)
bpul (laccase from Bacillus pumilus) with T7 promoter, RBS and HIS tag

BBa_K863001
BBa_K863001 Version 1 (Component)
bpul laccase from Bacillus pumilus

BBa_K863010
BBa_K863010 Version 1 (Component)
tthl laccase from Thermus thermophilus with T7 promoter, RBS and His-tag

BBa_K863011
BBa_K863011 Version 1 (Component)
tthl laccase from Thermus thermophilus

BBa_K861070
BBa_K861070 Version 1 (Component)
AdrA gene from E.coli K12

BBa_K861090
BBa_K861090 Version 1 (Component)
YhjH Gene From <i>E.coli str. K12</i>

BBa_K863030
BBa_K863030 Version 1 (Component)
tvel5 laccase cDNA sequence from Trametes versicolor

BBa_K863012
BBa_K863012 Version 1 (Component)
tthl laccase from T. thermophilus with constitutive promoter J23100, RBS and His-tag

BBa_K863034
BBa_K863034 Version 1 (Component)
pcil35 laccase cDNA sequence from Pycnoporus cinnabarinus

LipA
BBa_K836000 Version 1 (Component)
lipA from B. cepacia (codon usage optimized for E. coli)

DGAT
BBa_K836001 Version 1 (Component)
O-acyltransferase WSD from Acinetobacter sp. (codon usage optimized for R. opacus)

DGAT
BBa_K836002 Version 1 (Component)
O-acyltransferase WSD from Acinetobacter sp. (codon usage optimized for R. opacus) as used

lipA
BBa_K836003 Version 1 (Component)
lipA from B. cepacia (codon usage optimized for E. coli)

BBa_K759003
BBa_K759003 Version 1 (Component)
phaA (beta-ketothiolase orgin from Ralstonia eutropha)

BBa_K759004
BBa_K759004 Version 1 (Component)
phaA (beta-ketothiolase orgin from Ralstonia eutropha)

BBa_K863020
BBa_K863020 Version 1 (Component)
bhal laccase from Bacillus halodurans with T7 promoter, RBS and His-tag

BBa_K759005
BBa_K759005 Version 1 (Component)
phaB (acetoacetyl-CoA reductase orgin from Ralstonia eutropha)

BBa_K759006
BBa_K759006 Version 1 (Component)
B0034 and phaA (beta-ketothiolase orgin from Ralstonia eutropha)

gpS
BBa_K836004 Version 1 (Component)
Lysis inhibitor from Enterobacteria phage lambda (codon usage optimized for R. opacus)

BBa_K836005
BBa_K836005 Version 1 (Component)
Lysozyme from Rhodococcus phage RER2 (codon usage optimized for R, opacus)

BBa_K759007
BBa_K759007 Version 1 (Component)
RBS(B0034)and phaB (acetoacetyl-CoA reductase orgin from Ralstonia eutropha)

gpS
BBa_K836006 Version 1 (Component)
Lysis protein S from Enterobacteria phage lambda (codon usage optimized for R. opacus)

nitR
BBa_K836007 Version 1 (Component)
Nitrilase regulator from R. rhodochrous (codon usage optimized for R. opacus)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K102906
BBa_K102906 Version 1 (Component)
TA8 gate from synthetic algorithm v1.1

BBa_K102907
BBa_K102907 Version 1 (Component)
TA9 gate from synthetic algorithm v1.1

BBa_K102908
BBa_K102908 Version 1 (Component)
TA10 gate from synthetic algorithm v1.1

BBa_K102909
BBa_K102909 Version 1 (Component)
TA11 gate from synthetic algorithm v1.1

BBa_K102910
BBa_K102910 Version 1 (Component)
TA12 gate from synthetic algorithm v1.1

BBa_K102911
BBa_K102911 Version 1 (Component)
TA13 gate from synthetic algorithm v1.2