BBa_K176093
BBa_K176093 Version 1 (Component)
Cell density controller: pCon 1.00->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)

BBa_K176094
BBa_K176094 Version 1 (Component)
Cell density controller: pCon 0.70->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)

BBa_K176092
BBa_K176092 Version 1 (Component)
Cell density controller: pCon max->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)

BBa_K142046
BBa_K142046 Version 1 (Component)
tet-controlled LacI generator

BBa_J04795
BBa_J04795 Version 1 (Component)
Riboswitch designed to turn "ON" a protein

BBa_K176199
BBa_K176199 Version 1 (Component)
Cell density controller: pCon 0.70->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB-LVA(weak RBS)

BBa_K176215
BBa_K176215 Version 1 (Component)
Cell density controller: pCon 0.01->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB-LVA(weak RBS)

BBa_K176196
BBa_K176196 Version 1 (Component)
Cell density controller: pCon 0.70->luxI-LVA+pCon 0.36->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)

BBa_K176211
BBa_K176211 Version 1 (Component)
Cell density controller: pCon 0.04->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB-LVA(weak RBS)

BBa_K176207
BBa_K176207 Version 1 (Component)
Cell density controller: pCon 0.15->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB-LVA(weak RBS)

BBa_K176153
BBa_K176153 Version 1 (Component)
Cell density controller: pCon 0.04->luxI-LVA+pCon 0.36->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)

BBa_K176203
BBa_K176203 Version 1 (Component)
Cell density controller: pCon 0.36->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB-LVA(weak RBS)

BBa_K176150
BBa_K176150 Version 1 (Component)
Cell density controller: pCon 0.15->luxI-LVA+pCon 0.36->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)

BBa_K176147
BBa_K176147 Version 1 (Component)
Cell density controller: pCon 0.36->luxI-LVA+pCon 0.36->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)

BBa_K176156
BBa_K176156 Version 1 (Component)
Cell density controller: pCon 0.01->luxI-LVA+pCon 0.36->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)

BBa_K176204
BBa_K176204 Version 1 (Component)
Cell density controller: pCon 0.36->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA(weak RBS)

BBa_K176200
BBa_K176200 Version 1 (Component)
Cell density controller: pCon 0.70->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA(weak RBS)

BBa_K176208
BBa_K176208 Version 1 (Component)
Cell density controller: pCon 0.15->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA(weak RBS)

BBa_K176212
BBa_K176212 Version 1 (Component)
Cell density controller: pCon 0.04->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA(weak RBS)

BBa_K176216
BBa_K176216 Version 1 (Component)
Cell density controller: pCon 0.01->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA(weak RBS)

BBa_I763003
BBa_I763003 Version 1 (Component)
GFP coding device switched on by IPTG

placIQ RBS
BBa_K193604 Version 1 (Component)
GFP behind a constitutive promoter (placIQ) on pSB4A5

BBa_K1713016
BBa_K1713016 Version 1 (Component)
pTetR+B0034+LuxR(+MCS)+B0015

BBa_K249000
BBa_K249000 Version 1 (Component)
Tet Repressible YFP

BBa_J37021
BBa_J37021 Version 1 (Component)
pTetR Transfer Function with aTc

Stalker
BBa_J06001 Version 1 (Component)
Tet repressible luxI generator ( LVA+ )

BBa_I13110
BBa_I13110 Version 1 (Component)
Tet Self-coupled Inverter

placIQ RBS
BBa_K193601 Version 1 (Component)
Constitutive Promoter (placIQ ) + RBS + melA on Low copy vector(pSB6A1)

BBa_I759033
BBa_I759033 Version 1 (Component)
cis1-repressed, tet-regulated YFP

BBa_I759045
BBa_I759045 Version 1 (Component)
cis7-repressed, tet-regulated YFP

BBa_I759043
BBa_I759043 Version 1 (Component)
cis6-repressed, tet-regulated YFP

BBa_I759037
BBa_I759037 Version 1 (Component)
cis3-repressed, tet-regulated YFP

BBa_I759047
BBa_I759047 Version 1 (Component)
cis8-repressed, tet-regulated YFP

BBa_I759041
BBa_I759041 Version 1 (Component)
cis5-repressed, tet-regulated YFP

BBa_I759035
BBa_I759035 Version 1 (Component)
cis2-repressed, tet-regulated YFP

BBa_K165100
BBa_K165100 Version 1 (Component)
Gli1 bs + LexA bs + mCYC + LexA repressor (mCherryx2 tagged) on pRS304*

BBa_K1444011
BBa_K1444011 Version 1 (Component)
Composite promoter and consensus B. subtilis RBS - pTetR

BBa_S03736
BBa_S03736 Version 1 (Component)
pLac-lox-RBS-Tet (in pSB1A2)

BBa_K208013
BBa_K208013 Version 1 (Component)
Tet Repressible Promoter (BBa_R0040) and RBS (BBa_B0034)

BBa_K1444014
BBa_K1444014 Version 1 (Component)
Composite promoter and weak B. subtilis RBS - pTetR x2

BBa_S03766
BBa_S03766 Version 1 (Component)
RBS-Kan-RBS-Tet-RBS-RFP (pSB1A7)

BBa_K165101
BBa_K165101 Version 1 (Component)
Zif268-HIV bs + LexA bs + mCYC + Zif268-HIV repressor (mCherryx2 tagged) on pRS304*

BBa_K092500
BBa_K092500 Version 1 (Component)
TetR cds with RBS and Terminator + pTetR, RFP with RBS

BBa_S03737
BBa_S03737 Version 1 (Component)
pLac-lox-RFP(reverse)-TT-lox-RBS-Tet (psB1A2)

BBa_K415011
BBa_K415011 Version 1 (Component)
PtetR : RBS : LuxR : Term : PluxR/cI-OR : RBS : mCherry : Term : Plux/cI-OR : RBS : LuxI

BBa_K1520509
BBa_K1520509 Version 1 (Component)
PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.