BBa_K2066521
BBa_K2066521 Version 1 (Component)
UNS 6.2

BBa_K942009
BBa_K942009 Version 1 (Component)
SS+ Zea m 14+His

BBa_K2066021
BBa_K2066021 Version 1 (Component)
UNS 5 Sequence, from Torella et al., 2013

BBa_K2066539
BBa_K2066539 Version 1 (Component)
UNS 3.1

BBa_K2066540
BBa_K2066540 Version 1 (Component)
UNS 3.2

BBa_K2066507
BBa_K2066507 Version 1 (Component)
UNS 6.1 (Torella et. al 2013)

BBa_J23018
BBa_J23018 Version 1 (Component)
EN-Ptet-rbs-X-[RFP]-SNP "ORF Expresser"

BBa_K2066020
BBa_K2066020 Version 1 (Component)
UNS 4 Sequence, from Torella et al., 2013

STS
BBa_K1825001 Version 1 (Component)
Stilbene synthase (STS) from grapes

BBa_K1100019
BBa_K1100019 Version 1 (Component)
R1062 with insulator and sfGFP

BBa_K1100016
BBa_K1100016 Version 1 (Component)
R0040 with insulator and sfGFP

BBa_K1100015
BBa_K1100015 Version 1 (Component)
R0010 with insulator and sfGFP

BBa_K1100105
BBa_K1100105 Version 1 (Component)
J23105 with insulator and sfGFP

BBa_K1100018
BBa_K1100018 Version 1 (Component)
R1051 with insulator and sfGFP

BBa_K1100104
BBa_K1100104 Version 1 (Component)
J23104 with insulator and sfGFP

BBa_K1100101
BBa_K1100101 Version 1 (Component)
J23101 with insulator and sfGFP

BBa_K1100110
BBa_K1100110 Version 1 (Component)
J23110 with insulator and sfGFP

BBa_K1100100
BBa_K1100100 Version 1 (Component)
J23100 with insulator and sfGFP

BBa_K1100017
BBa_K1100017 Version 1 (Component)
R0063 with insulator and sfGFP

BBa_I766002
BBa_I766002 Version 1 (Component)
YopJ, Irrev. MAPKK Ser/Thr Acetylase

BBa_K590082
BBa_K590082 Version 1 (Component)
Ser-Glylinker

Sen
BBa_I726041 Version 1 (Component)
Sen-TIC

SET
BBa_K1433011 Version 1 (Component)
Terminator-RFP-RBS-attB-P-attP-RBS-GFP-Terminator

Sen
BBa_I726051 Version 1 (Component)
Sen-RIC

BBa_K2066541
BBa_K2066541 Version 1 (Component)
UNS 2.1

BBa_K2066542
BBa_K2066542 Version 1 (Component)
UNS 2.2

BBa_K1639004
BBa_K1639004 Version 1 (Component)
H-NS

BBa_K2066522
BBa_K2066522 Version 1 (Component)
64BP Spacer, UNS X + first 24 UNS X

BBa_K2066514
BBa_K2066514 Version 1 (Component)
16bp spacer from UNS X

BBa_K1418041
BBa_K1418041 Version 1 (Component)
CFP(ns)

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

LongLinker
BBa_K243006 Version 1 (Component)
Long Linker (Gly-Gly-Ser-Gly)x3

BBa_K1148012
BBa_K1148012 Version 1 (Component)
4CL::STS

BBa_I766336
BBa_I766336 Version 1 (Component)
Ser-Gly-Ser-Gly-Ser ING2 PH Domain

MiddleLink
BBa_K243005 Version 1 (Component)
Middle Linker ( Gly-Gly-Ser-Gly)x2

BBa_M1189
BBa_M1189 Version 1 (Component)
ssn