BBa_K411123
BBa_K411123 Version 1 (Component)
Split RFP-eIF4A_A Generator (R0040 + B0034 + RFP_split_A+Linker+eIF4A_split_A + B0015)

VVD-YN
BBa_K1616002 Version 1 (Component)
VVD linked to YN155 (YFP Nter split) with double terminator T7

BBa_K1053124
BBa_K1053124 Version 1 (Component)
Split EYFP C-terminal domain fused with λN peptide

BBa_K411124
BBa_K411124 Version 1 (Component)
Split RFP-eIF4A_B Generator (R0040 + B0034 + RFP_split_B+Linker+eIF4A_split_B + B0015)

BBa_K1053123
BBa_K1053123 Version 1 (Component)
Split EYFP N-terminal domain fused with HTLV-1 Rex peptide

BBa_K323005
BBa_K323005 Version 1 (Component)
CMV ATG PBSII link nYFP stop - part of mammalian split/FRET system

BBa_K323021
BBa_K323021 Version 1 (Component)
CMV ATG Gli1 link nCFP stop - part of mammalian split/FRET system

BBa_J72204
BBa_J72204 Version 1 (Component)
ampicillin resistant split R6K vector w/ O16 HAs and FRT-Kan-FRT

BBa_K738004
BBa_K738004 Version 1 (Component)
FA-2X-MS2: Split GFP N-terminal domain fused with MS2 protein

BBa_K738005
BBa_K738005 Version 1 (Component)
FB-2X-PP7: Split GFP C-terminal domain fused with PP7 protein

BBa_K323080
BBa_K323080 Version 1 (Component)
CMV ATG cYFP link Zif268 link His stop - part of mammalian split/FRET system

BBa_K323029
BBa_K323029 Version 1 (Component)
CMV ATG cCFP link ZNF HIVC His stop - part of mammalian split/FRET system

BBa_K1616003
BBa_K1616003 Version 1 (Component)
VVD link to YC155 (YFP Cter split)

BBa_K1616004
BBa_K1616004 Version 1 (Component)
VVD linked to YN155 (YFP Nter split)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.