BBa_K1485002
BBa_K1485002 Version 1 (Component)
A kind of GFP for eukaryotes with a linker (GS)x5

BBa_K299800
BBa_K299800 Version 1 (Component)
standard biobrick scar (if next part starts with ATG)

CBDcex(T7)
BBa_K863102 Version 1 (Component)
Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)

BBa_J61207
BBa_J61207 Version 1 (Component)
TrnspsnPlsmd Tn5F|rbsGenR|SpecR|P_T7|Tn5R||CmR|TnP|OriTr|R6K

BBa_K180020
BBa_K180020 Version 1 (Component)
Rock-paper-scissors - Scissors secondary plasmid [las pR, LacI generator]

BBa_K180013
BBa_K180013 Version 1 (Component)
Rock-paper-scissors - Rock secondary plasmid [rhl pR, LacI generator]

BBa_K133102
BBa_K133102 Version 1 (Component)
CMV-SS-HA-UreB-CD4sh-(TIR)TLR3-(TIR)TLR9-Histop

BBa_K133136
BBa_K133136 Version 1 (Component)
CMV-SS-HA-UreB-gyrEC-linker-(TMTIR)TLR9-GFP-Histop

BBa_J61212
BBa_J61212 Version 1 (Component)
Tn5F|rbsGenR|FRT|R6K|CmR|FRT|P_T7|Tn5R||SpecR|OriTr|TnP

BBa_K1456012
BBa_K1456012 Version 1 (Component)
Human Tissue Plasminogen Activator reverse primer with restriction site, 2 stop codons and 6X His

BBa_K1695013
BBa_K1695013 Version 1 (Component)
Riboswitch + Bacteriophage 21 codon optimized R gene

BBa_K1695014
BBa_K1695014 Version 1 (Component)
Riboswitch + Bacteriophage 21 codon optimized Rz gene

BBa_K1695012
BBa_K1695012 Version 1 (Component)
Riboswitch + Bacteriophage 21 codon optimized S gene

BBa_K180015
BBa_K180015 Version 1 (Component)
Paper - Primary plasmid (part 2) [tac pR, GFP and RhlI generator]

BBa_K1456024
BBa_K1456024 Version 1 (Component)
Human Tissue Plasminogen Activator reverse primer-2 with restriction site, 2 stop codons and 6XHis

BBa_K1974033
BBa_K1974033 Version 1 (Component)
T7 Promoter+RBS+Hv1a+GS linker+snowdrop-lectin+linker+6X His-Tag

BBa_K1162307
BBa_K1162307 Version 1 (Component)
WAM-1 antimicrobial peptide fused with 8x spider silk protein, with 10x His-Tag

BBa_K371055
BBa_K371055 Version 1 (Component)
MPF(meta-prefix)+[GFP+10*GS+A] fusion proterin +MSF(meta-suffix)

BBa_K371031
BBa_K371031 Version 1 (Component)
MPF(meta-prefix)+[pduV98+10*GS+GFP] fusion protein+MSF(meta-suffix)

BBa_K371030
BBa_K371030 Version 1 (Component)
MPF(meta-prefix)+[pduP64+10*GS+GFP] fusion protein+MSF(meta-suffix)

BBa_K2012009
BBa_K2012009 Version 1 (Component)
RBS(J61100) and CheZ with fused with a GS linker and GFP in C teminal

BBa_K180005
BBa_K180005 Version 1 (Component)
GoL - Primary plasmid (part 1)/RPS - Paper primary plasmid (part 1) [LuxR generator]

BBa_K1051356
BBa_K1051356 Version 1 (Component)
K1051301(clb2 promoter) + K1051053(K1051001 (non stop codon ECFP + K1051006 (Stop codon + TBY-1 term

BBa_K2012021
BBa_K2012021 Version 1 (Component)
PcpcG2-172-B0034-CheZ-GS3-GFP

BBa_K942003
BBa_K942003 Version 1 (Component)
SS+B.P.S.

BBa_K371024
BBa_K371024 Version 1 (Component)
10*GS linker(for RFC 53 protein fusion)

BBa_K942016
BBa_K942016 Version 1 (Component)
SS+B.P.S.

BBa_K942017
BBa_K942017 Version 1 (Component)
SS+B.P.S.

BBa_K942019
BBa_K942019 Version 1 (Component)
SS+B.P.S.

pSBBs0K
BBa_K823026 Version 1 (Component)
pSB_Bs0K-P_spac (replicative Bacillus subtilis expression vector; IPTG inducible

BBa_K2172009
BBa_K2172009 Version 1 (Component)
Tac Promoter-RBS-GST-Thrombin Protease-GFP-Terminator

BBa_K1695042
BBa_K1695042 Version 1 (Component)
Riboswitch Bacteriophage 21 Codon Optimized Lysis Cassette S R Rz

GG100
BBa_K2145125 Version 1 (Component)
This part contains 2 fluorescent protein coding sites (RFP and GFP) with a spacer

GG98
BBa_K2145123 Version 1 (Component)
This part contains 2 fluorescent protein coding sites (RFP and GFP) with a spacer

BBa_K133175
BBa_K133175 Version 1 (Component)
CMV-SS-gyrEC

BBa_K133172
BBa_K133172 Version 1 (Component)
CMV-SS-gyrHP

BBa_K1695049
BBa_K1695049 Version 1 (Component)
pL8-UV5 + Riboswitch Bacteriophage 21 Codon Optimized Lysis Cassette S R Rz

BBa_K133023
BBa_K133023 Version 1 (Component)
CMV-ss-CF213-multiHP-CF215-RGD-Histop (term)

BBa_K371054
BBa_K371054 Version 1 (Component)
MPF(meta-prefix)+[GFP+10*GS+A] fusion protein+MSF(meta-suffix))

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.