BBa_K1974033
BBa_K1974033 Version 1 (Component)
T7 Promoter+RBS+Hv1a+GS linker+snowdrop-lectin+linker+6X His-Tag

BBa_K1824556
BBa_K1824556 Version 1 (Component)
RNA Thermometer A1 (Specially designed for J23119)

BBa_K1824559
BBa_K1824559 Version 1 (Component)
RNA Thermometer FourU (Specially designed for J23119)

BBa_K549004
BBa_K549004 Version 1 (Component)
LacI promotor fused with the iron dependent regulator fur

BBa_I741016
BBa_I741016 Version 1 (Component)
Reverse Total XylR Transcriptional Regulator Left Facing (I741011 reverse complement

BBa_K1615108
BBa_K1615108 Version 1 (Component)
mRFP fused to CBDclos driven by LacI promoter

BBa_K1405007
BBa_K1405007 Version 1 (Component)
A Kill Switch with "memory" time repressed by IPTG

PLac-LacY-
BBa_I763013 Version 1 (Component)
LacY and cI coding device switched on by IPTG

BBa_K145274
BBa_K145274 Version 1 (Component)
LuxI generator controlled by a AND-gate system.

BBa_K258003
BBa_K258003 Version 1 (Component)
Granulysin, a T Cell Product,Kills Bacteria by Altering Membrane Permeability

BBa_K1363604
BBa_K1363604 Version 1 (Component)
key of no of RNA logic gates

BBa_K395102
BBa_K395102 Version 1 (Component)
GFP reporter repressed by LuxR and 3OC6HSL (K395005:K121013)

BBa_K395103
BBa_K395103 Version 1 (Component)
GFP reporter repressed by LuxR and 3OC6HSL (K395006:K121013)

BBa_J58014
BBa_J58014 Version 1 (Component)
Promoter activated by OmpR-P with the reporter GFP

BBa_K290001
BBa_K290001 Version 1 (Component)
constitutive RhlR with bicistronic LuxI - GFP controlled by pRhl

BBa_J58015
BBa_J58015 Version 1 (Component)
Mutated promoter activated by OmpR-P with the reporter GFP

BBa_K779117
BBa_K779117 Version 1 (Component)
RNA Reporter top strand with quencher (RQ) MammoBlock

BBa_K300096
BBa_K300096 Version 1 (Component)
Double phasin and intein separed by a flexible protein domain linker

BBa_K779116
BBa_K779116 Version 1 (Component)
Short RNA reporter bottom strand (with ROX fluorophore) MammoBlock

BBa_K779120
BBa_K779120 Version 1 (Component)
RNA Reporter top strand with quencher (RQ) and tag fluorophore (Alexa 488) MammoBlock

BBa_K1942001
BBa_K1942001 Version 1 (Component)
This part is a short RNA sequence designed for KRAS gene silencing. It is used for down-regulating K

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.