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BBa_K145274

BBa_K145274 Version 1

Component

This part has been discontinued.

Please consider using BBa_K145104 as a replacement.

Source:
http://parts.igem.org/Part:BBa_K145274

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nathalie Busschaert

Date created: 2008-09-06 11:00:00

Date modified: 2015-05-08 01:10:29

LuxI generator controlled by a AND-gate system.

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Details

Types	DnaRegion
Roles	Generator engineered_region
Sequences	BBa_K145274_sequence (Version 1)

Description

LuxI is placed under a LacI repressible promoter. The production of lacI itself is controlled by a T7 promoter and by a ribolock. Only when both T7 DNA polymerase and ribokey3d are present, will lacI be produced. In that case the production of LuxI is repressed. When none or only one of the regulators is present, lacI will not be produced and the production of LuxI is switched on.

Components

Access	Instance	Definition
public public public public public public public public public public	BBa_I712074 BBa_J23032 BBa_C0012 BBa_B0010 BBa_B0012 BBa_R0011 BBa_B0032 BBa_C0061 BBa_B0010 BBa_B0012	BBa_I712074 BBa_J23032 lacI BBa_B0010 BBa_B0012 lacI+pL BBa_B0032 luxI BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I712074 BBa_J23032 BBa_C0012 BBa_B0010 BBa_B0012 BBa_R0011 BBa_B0032 BBa_C0061 BBa_B0010 BBa_B0012	1,46 55,97 104,1231 1265,1344 1353,1393 1402,1455 1465,1477 1484,2101 2135,2214 2223,2263	BBa_I712074 BBa_J23032 lacI BBa_B0010 BBa_B0012 lacI+pL BBa_B0032 luxI BBa_B0010 BBa_B0012

Other Properties

igem#experience	None
igem#sampleStatus	Discontinued
igem#status	Deleted
isReplacedBy	BBa_K145104/1
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K145274/1