BBa_I716201
BBa_I716201 Version 1 (Component)
[rbs][ATG]

BBa_I716203
BBa_I716203 Version 1 (Component)
[rbs][ATG]

BBa_I716202
BBa_I716202 Version 1 (Component)
[rbs][ATG]

BBa_K784035
BBa_K784035 Version 1 (Component)
T7*(N4) RNA polymerase

RBS
BBa_K1893033 Version 1 (Component)
Strong ribosome binding site (RBS)

BBa_K274100
BBa_K274100 Version 1 (Component)
CrtEBI with rbs

MotB
BBa_S03271 Version 1 (Component)
MotB: RBS+flagellar protein

BBa_K1021004
BBa_K1021004 Version 1 (Component)
T7 promoter + RBS + afp1 (antifungal)

BBa_K797008
BBa_K797008 Version 1 (Component)
Plasmid include T7-RBS-R9 region

Strep-tag
BBa_M0031 Version 1 (Component)
Strep-tag II affinity tag

BBa_C7077
BBa_C7077 Version 1 (Component)
T7 gene 7.7

BBa_M36009
BBa_M36009 Version 1 (Component)
5' Bicistronic UTR (strong), does not include ATG start

BBa_K215002
BBa_K215002 Version 1 (Component)
pLac+RBS+Secretion Signal and Streptavidin Binding Tags

BBa_K823035
BBa_K823035 Version 1 (Component)
HA-tag (Freiburg standard+RBS)

BBa_K823036
BBa_K823036 Version 1 (Component)
cMyc-tag (Freiburg standard+RBS)

BBa_K1162010
BBa_K1162010 Version 1 (Component)
promoter+rbs and N-terminal 10x His Tag

BBa_K1399006
BBa_K1399006 Version 1 (Component)
GFP (mut3b) with LAA-ssrA degradation tag

BBa_K1399008
BBa_K1399008 Version 1 (Component)
GFP (mut3b) with DAS-ssrA degradation tag

BBa_K1362444
BBa_K1362444 Version 1 (Component)
GGG linker with RGK (extein)

BBa_K176063
BBa_K176063 Version 1 (Component)
PoPS->RBS(weak)+lacZalpha-ccdB+T

BBa_K176062
BBa_K176062 Version 1 (Component)
PoPS->RBS(weak)+ccdB+T

BBa_K1435000
BBa_K1435000 Version 1 (Component)
4-HT inducible intein

BBa_K1806003
BBa_K1806003 Version 1 (Component)
T7+ ibPB RNA Thermometer + lacI

BBa_K1806005
BBa_K1806005 Version 1 (Component)
T7+ ibPB RNA Thermometer + RFP

BBa_K1758122
BBa_K1758122 Version 1 (Component)
RraA with N-terminal His-TEV-tag under T7 promoter

BBa_I715040
BBa_I715040 Version 1 (Component)
pLac-RBS-RFP-RBS-GFP

BBa_K228250
BBa_K228250 Version 1 (Component)
T7 promoter->RBS->cI->Terminator

BBa_I715038
BBa_I715038 Version 1 (Component)
pLac-RBS-T7 RNA Polymerase

BBa_K1092011
BBa_K1092011 Version 1 (Component)
T7-RBS-ssDsbA-PDZ Ligand

BBa_K228248
BBa_K228248 Version 1 (Component)
T7 promoter->RBS->cI->Terminator

BBa_K228249
BBa_K228249 Version 1 (Component)
T7 promoter->RBS->cI->Terminator

BBa_K228251
BBa_K228251 Version 1 (Component)
T7 promoter->RBS->cI->Terminator

BBa_K228252
BBa_K228252 Version 1 (Component)
T7 promoter->RBS->cI->Terminator

BBa_K228253
BBa_K228253 Version 1 (Component)
T7 promoter->RBS->cI->Terminator

BBa_K228254
BBa_K228254 Version 1 (Component)
T7 promoter->RBS->cI->Terminator

BBa_K228247
BBa_K228247 Version 1 (Component)
T7 promoter->RBS->cI->Terminator

BBa_K228246
BBa_K228246 Version 1 (Component)
T7 promoter->RBS->cI->Terminator

BBa_K1123012
BBa_K1123012 Version 1 (Component)
His-Tag and Thrombine Cleavage Site

BBa_K1179069
BBa_K1179069 Version 1 (Component)
CAG promoter

BBa_J70628
BBa_J70628 Version 1 (Component)
RFC12 Sce VMA Intein Internal Domain

BBa_K180000
BBa_K180000 Version 1 (Component)
Hybrid promoter (trp & lac regulated -- tac pR)

BBa_K360121
BBa_K360121 Version 1 (Component)
RBS + LovTAP Photosensor + terminator

BBa_K1375024
BBa_K1375024 Version 1 (Component)
Prhl/las +RBS

BBa_K1676005
BBa_K1676005 Version 1 (Component)
RBS Mutant 2AG

BBa_K1676002
BBa_K1676002 Version 1 (Component)
RBS Mutant 1AG

BBa_K1676008
BBa_K1676008 Version 1 (Component)
RBS Mutant 3AG

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1044007
BBa_K1044007 Version 1 (Component)
T7 promoter (strong promoter from T7 bacteriophage)+RBS (Elowitz 1999) -- defines RBS efficiency