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Showing 3251 - 3292 of 3292 result(s)
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Public
BBa_I12029
BBa_I12029 Version 1 (Component)
Test of pBAD/araC promoter (I0500)
Public
BBa_K1707004
BBa_K1707004 Version 1 (Component)
TetR-ssRA repressor with the promoter of cI
Public
RBS-TetR-T
BBa_K079007 Version 1 (Component)
TetR protein with terminator and RBS
Public
BBa_K1140006
BBa_K1140006 Version 1 (Component)
pTet + 37 oC RNA thermometer + mCherry (LVA)
Public
BBa_K820014
BBa_K820014 Version 1 (Component)
mazF-TT
Public
iGEM 2019 Cell Tat secretion cassette with constitutive promoter.
iGEM_2019_Cell17 Version 1 (Collection)

Public
BBa_K329003
BBa_K329003 Version 1 (Component)
Strong RBS (B0034) - Tetracycline resistance protein TetA(C) (J31007)
Public
BBa_K2017010
BBa_K2017010 Version 1 (Component)
35s + Ga20ox consense + RSIAT-TEV-Luciferase + Tnos
Public
BBa_K175018
BBa_K175018 Version 1 (Component)
GFP+TT
Public
BBa_I12026
BBa_I12026 Version 1 (Component)
Test of BBa_R0011 (LacI regulated) using YFP
Public
BBa_K315045
BBa_K315045 Version 1 (Component)
pTet+loxP forward+RBS+RFP+loxN forward
Public
BBa_J31016
BBa_J31016 Version 1 (Component)
part produces the RNA construct crRNA-RBS-GFPLVA-tt that can only be translated in the presence of t
Public
BBa_S03641
BBa_S03641 Version 1 (Component)
HixC : TetB-RBS<sub>rev</sub>-HixC
Public
BBa_K091119
BBa_K091119 Version 1 (Component)
LacI protein generator with a pTet promoter
Public
BBa_K876016
BBa_K876016 Version 1 (Component)
plac-lacO-RBS-GFP-T-pTet-LacI
Public
BBa_I13901
BBa_I13901 Version 1 (Component)
Synchronized Brickilator Test Construct (I13201.Q04400.I0461)
Public
AraC_TEV-F
BBa_K627010 Version 1 (Component)
Fusion of AraC induction system and TEV protease 3
Public
BBa_K199112
BBa_K199112 Version 1 (Component)
RFP-TT intermediate
Public
BBa_J69122
BBa_J69122 Version 1 (Component)
C12 AHL Receiver test construct with EYFP Reporter
Public
BBa_S03638
BBa_S03638 Version 1 (Component)
HixC-pBad<sub>rev</sub>-HixC : RBS-TetF
Public
BBa_K228816
BBa_K228816 Version 1 (Component)
Promoter(constitutive)+RBS(B0034)+tetR(C0040)+terminator(B0015)
Public
BBa_K516222
BBa_K516222 Version 1 (Component)
AiiA protein generator with Ptet and RBS B0032
Public
BBa_K1088058
BBa_K1088058 Version 1 (Component)
10 aa linker with BamHI restriction site and TEV recognition site
Public
BBa_K1638006
BBa_K1638006 Version 1 (Component)
10 aa linker with BamHI restriction site and TEV recognition site
Public
BBa_K660605
BBa_K660605 Version 1 (Component)
OmpF + wYFP + TT
Public
BBa_K101013
BBa_K101013 Version 1 (Component)
Construct with TetR/p22cII promoter, RBS, RFP, Terminator
Public
BBa_K101012
BBa_K101012 Version 1 (Component)
Construct with TetR/p22cII promoter, RBS, RFP, Terminator
Public
BBa_K831006
BBa_K831006 Version 1 (Component)
Inducible MqsR toxin under the control of tetR promoter
Public
BBa_K196013
BBa_K196013 Version 1 (Component)
Hybrid promoter (Lux cassette + c2 P22 promoter) + RBS + LuxR + ter
Public
BBa_K332034
BBa_K332034 Version 1 (Component)
Constitutive promoter + 37&#8451; induced RBS + tetR + double terminator
Public
BBa_K1172914
BBa_K1172914 Version 1 (Component)
Part 2 of the Biosafety-System TetOR alive (TetO GFP)
Public
AraC_TEV-F
BBa_K627008 Version 1 (Component)
Fusion part of arabinose-inducible induction system and the TEV protease
Public
BBa_S03674
BBa_S03674 Version 1 (Component)
(-1,2) HixC-pBad<sub>rev</sub>-HixC : RBS-TetF-HixC
Public
BBa_K092500
BBa_K092500 Version 1 (Component)
TetR cds with RBS and Terminator + pTetR, RFP with RBS
Public
BBa_K318501
BBa_K318501 Version 1 (Component)
lacI pL + RBS + RcsB + TT
Public
[OriTR]+[R
BBa_K1439002 Version 1 (Component)
This part contains a reporter gene BBa_J04450, combined with OriTR. Used to test plasmid mobility.
Public
BBa_K1067000
BBa_K1067000 Version 1 (Component)
Periplasmic directed GFP SF with signal peptide TAT and RFP as background color
Public
BBa_I13006
BBa_I13006 Version 1 (Component)
Promoter R0040 w/ YFP (-LVA) TT
Public
BBa_K415005
BBa_K415005 Version 1 (Component)
pLux/cI-OR : RBS-mCherry : Term : p(tetR) : RBS-luxR : Term
Public
BBa_K199165
BBa_K199165 Version 1 (Component)
pT7 + RBS + GFP + TT in pSB3T5
Public
BBa_K2066500
BBa_K2066500 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013
Public
Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
Showing 3251 - 3292 of 3292 result(s)
Previous 61 62 63 64 65 66