BBa_I12029BBa_I12029 Version 1 (Component)Test of pBAD/araC promoter (I0500)
BBa_K1707004BBa_K1707004 Version 1 (Component)TetR-ssRA repressor with the promoter of cI
RBS-TetR-TBBa_K079007 Version 1 (Component)TetR protein with terminator and RBS
BBa_K1140006BBa_K1140006 Version 1 (Component)pTet + 37 oC RNA thermometer + mCherry (LVA)
BBa_K329003BBa_K329003 Version 1 (Component)Strong RBS (B0034) - Tetracycline resistance protein TetA(C) (J31007)
BBa_K2017010BBa_K2017010 Version 1 (Component)35s + Ga20ox consense + RSIAT-TEV-Luciferase + Tnos
BBa_I12026BBa_I12026 Version 1 (Component)Test of BBa_R0011 (LacI regulated) using YFP
BBa_K315045BBa_K315045 Version 1 (Component)pTet+loxP forward+RBS+RFP+loxN forward
BBa_J31016BBa_J31016 Version 1 (Component)part produces the RNA construct crRNA-RBS-GFPLVA-tt that can only be translated in the presence of t
BBa_S03641BBa_S03641 Version 1 (Component)HixC : TetB-RBS<sub>rev</sub>-HixC
BBa_K091119BBa_K091119 Version 1 (Component)LacI protein generator with a pTet promoter
BBa_K876016BBa_K876016 Version 1 (Component)plac-lacO-RBS-GFP-T-pTet-LacI
BBa_I13901BBa_I13901 Version 1 (Component)Synchronized Brickilator Test Construct (I13201.Q04400.I0461)
AraC_TEV-FBBa_K627010 Version 1 (Component)Fusion of AraC induction system and TEV protease 3
BBa_K199112BBa_K199112 Version 1 (Component)RFP-TT intermediate
BBa_J69122BBa_J69122 Version 1 (Component)C12 AHL Receiver test construct with EYFP Reporter
BBa_S03638BBa_S03638 Version 1 (Component)HixC-pBad<sub>rev</sub>-HixC : RBS-TetF
BBa_K228816BBa_K228816 Version 1 (Component)Promoter(constitutive)+RBS(B0034)+tetR(C0040)+terminator(B0015)
BBa_K516222BBa_K516222 Version 1 (Component)AiiA protein generator with Ptet and RBS B0032
BBa_K1088058BBa_K1088058 Version 1 (Component)10 aa linker with BamHI restriction site and TEV recognition site
BBa_K1638006BBa_K1638006 Version 1 (Component)10 aa linker with BamHI restriction site and TEV recognition site
BBa_K660605BBa_K660605 Version 1 (Component)OmpF + wYFP + TT
BBa_K101013BBa_K101013 Version 1 (Component)Construct with TetR/p22cII promoter, RBS, RFP, Terminator
BBa_K101012BBa_K101012 Version 1 (Component)Construct with TetR/p22cII promoter, RBS, RFP, Terminator
BBa_K831006BBa_K831006 Version 1 (Component)Inducible MqsR toxin under the control of tetR promoter
BBa_K196013BBa_K196013 Version 1 (Component)Hybrid promoter (Lux cassette + c2 P22 promoter) + RBS + LuxR + ter
BBa_K332034BBa_K332034 Version 1 (Component)Constitutive promoter + 37℃ induced RBS + tetR + double terminator
BBa_K1172914BBa_K1172914 Version 1 (Component)Part 2 of the Biosafety-System TetOR alive (TetO GFP)
AraC_TEV-FBBa_K627008 Version 1 (Component)Fusion part of arabinose-inducible induction system and the TEV protease
BBa_S03674BBa_S03674 Version 1 (Component)(-1,2) HixC-pBad<sub>rev</sub>-HixC : RBS-TetF-HixC
BBa_K092500BBa_K092500 Version 1 (Component)TetR cds with RBS and Terminator + pTetR, RFP with RBS
BBa_K318501BBa_K318501 Version 1 (Component)lacI pL + RBS + RcsB + TT
[OriTR]+[RBBa_K1439002 Version 1 (Component)This part contains a reporter gene BBa_J04450, combined with OriTR. Used to test plasmid mobility.
BBa_K1067000BBa_K1067000 Version 1 (Component)Periplasmic directed GFP SF with signal peptide TAT and RFP as background color
BBa_I13006BBa_I13006 Version 1 (Component)Promoter R0040 w/ YFP (-LVA) TT
BBa_K415005BBa_K415005 Version 1 (Component)pLux/cI-OR : RBS-mCherry : Term : p(tetR) : RBS-luxR : Term
BBa_K199165BBa_K199165 Version 1 (Component)pT7 + RBS + GFP + TT in pSB3T5
BBa_K2066500BBa_K2066500 Version 1 (Component)UNS 2 Sequence, from Torella et al., 2013
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.