SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K157002
BBa_K157002 Version 1 (Component)
Transmembrane region of the EGF-Receptor (ErbB-1)

BBa_K101016
BBa_K101016 Version 1 (Component)
Dually repressed promoter with sites for TetR and P22MNT binding

BBa_K143071
BBa_K143071 Version 1 (Component)
AmyE integratable PoPS generator (P43-gsiB) (with CmR)

BBa_K143072
BBa_K143072 Version 1 (Component)
AmyE integratable PoPS generator (P43-spoVG) (with CmR)

BBa_K143073
BBa_K143073 Version 1 (Component)
AmyE integratable PoPS generator (Pveg-gsiB) (with CmR)

BBa_K143074
BBa_K143074 Version 1 (Component)
AmyE integratable PoPS generator (Pveg-spoVG) (with CmR)

BBa_J69522
BBa_J69522 Version 1 (Component)
tetR-repressible promoter coupled with mCherry Reporter

BBa_K122016
BBa_K122016 Version 1 (Component)
4CL:STS with CYC1 terminator.

RBS-RFP
BBa_K093005 Version 1 (Component)
RFP with RBS

RBS-cI
BBa_K093006 Version 1 (Component)
cI repressor with RBS

oriTr/LacZ
BBa_K125350 Version 1 (Component)
oriTr with lac promoter and lacZ

oriT/LacZ
BBa_K125360 Version 1 (Component)
oriT with lac promoter and lacZ

BBa_K086001
BBa_K086001 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter

BBa_K086018
BBa_K086018 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24

BBa_K086002
BBa_K086002 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP

BBa_K086019
BBa_K086019 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24

BBa_K086003
BBa_K086003 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter

BBa_K086020
BBa_K086020 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24

BBa_K125810
BBa_K125810 Version 1 (Component)
slr2016 signal sequence + GFP fusion for secretion of GFP

BBa_K156009
BBa_K156009 Version 1 (Component)
OFP (orange fluorescent protein)

OriTR
BBa_J01003 Version 1 (Component)
OriT-R (Origin of transfer for the R-plasmid nic region)

BBa_K105000
BBa_K105000 Version 1 (Component)
pSB1A2_fusion

BBa_K142008
BBa_K142008 Version 1 (Component)
lacI IS mutant R197A with RBS and terminator

BBa_K142009
BBa_K142009 Version 1 (Component)
lacI IS mutant R197F with RBS and terminator

BBa_K142010
BBa_K142010 Version 1 (Component)
lacI IS mutant T276A with RBS and terminator

BBa_K142011
BBa_K142011 Version 1 (Component)
lacI IS mutant T276Fwith RBS and terminator

BBa_K142012
BBa_K142012 Version 1 (Component)
lacI IS mutant R197A T276A with RBS and terminator

BBa_K142013
BBa_K142013 Version 1 (Component)
lacI IS mutant R197A T276F with RBS and terminator

BBa_K142014
BBa_K142014 Version 1 (Component)
lacI IS mutant R197F T276A with RBS and terminator

BBa_K142015
BBa_K142015 Version 1 (Component)
lacI IS mutant R197F T276F with RBS and terminator

BBa_K142202
BBa_K142202 Version 1 (Component)
SceI with RBS (BBa_B0030) and double terminator (BBa_B0015)

BBa_K142203
BBa_K142203 Version 1 (Component)
T4 ligase with RBS (BBa_B0030) and double terminator (BBa_B0015)

BBa_K156024
BBa_K156024 Version 1 (Component)
RBS + OFP

BBa_K116510
BBa_K116510 Version 1 (Component)
GFP under control of pOmpf promoter(K116500+E0240)

BBa_K142047
BBa_K142047 Version 1 (Component)
tet-controlled LacI generator with constitutive TetR expression cassette

BBa_K142024
BBa_K142024 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197A) and TetR expression cassette

BBa_K142025
BBa_K142025 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197F) and TetR expression cassette

BBa_K142026
BBa_K142026 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (T276A) and TetR expression cassette

BBa_K142027
BBa_K142027 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (T276F) and TetR expression cassette

BBa_K142028
BBa_K142028 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197A, T276A)/TetR expression cassette

BBa_K142029
BBa_K142029 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197A, T276F)/TetR expression cassette

BBa_K142030
BBa_K142030 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197F, T276A)/TetR expression cassette

BBa_K142031
BBa_K142031 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197F, T276F)/TetR expression cassette

BBa_K116501
BBa_K116501 Version 1 (Component)
RpaA(regulator of phycobilisome-associated)

BBa_K116503
BBa_K116503 Version 1 (Component)
GFP under control of pOmpC promoter.R0082+E0240

BBa_K133083
BBa_K133083 Version 1 (Component)
CMV-SS-HA-UreB-gyrEC-2xlinker-(TMTIR)TLR9-Histop

BBa_K133093
BBa_K133093 Version 1 (Component)
CMV-SS-HA-UreB-CD4ecto

BBa_K133094
BBa_K133094 Version 1 (Component)
CMV-SS-HA-UreB-CD4ecto-(TMTIR)TLR9-Histop