BBa_J72068
BBa_J72068 Version 1 (Component)
prepro with degradation signal

BBa_M1016
BBa_M1016 Version 1 (Component)
PhaB with zif23-GCN4

BBa_J72069
BBa_J72069 Version 1 (Component)
prepro with degradation signal

BBa_J72066
BBa_J72066 Version 1 (Component)
prepro with degradation signal

BBa_K1017403
BBa_K1017403 Version 1 (Component)
sRNA-1 (with Plux)

BBa_K303002
BBa_K303002 Version 1 (Component)
Plac/ara with J23106

BBa_K303004
BBa_K303004 Version 1 (Component)
Ptet/lac with J23110

BBa_J100220
BBa_J100220 Version 1 (Component)
tCloneTet with Riboswitch 4

BBa_K303001
BBa_K303001 Version 1 (Component)
Ptet/lac with J23106

BBa_K879358
BBa_K879358 Version 1 (Component)
tetR with promoter 3

BBa_J100218
BBa_J100218 Version 1 (Component)
tCloneTet with Riboswitch 3shift

BBa_J119041
BBa_J119041 Version 1 (Component)
superfolder GFP with LVA

BBa_K176102
BBa_K176102 Version 1 (Component)
constitutive promoter family member K176009 (pCon 0.36) with primer VR7 site

BBa_M1019
BBa_M1019 Version 1 (Component)
Silver Fusion Compatible PhaB gene with zif23-GCN4

BBa_K519012
BBa_K519012 Version 1 (Component)
Promoter and RBS with PduP1~18 tag

BBa_K091207
BBa_K091207 Version 1 (Component)
S04169 with S04165

BBa_K077020
BBa_K077020 Version 1 (Component)
ptet with LasR

BBa_M1733
BBa_M1733 Version 1 (Component)
Hepcidin with GFP

BBa_K091208
BBa_K091208 Version 1 (Component)
S04169 with S04167

rbs+sam5
BBa_I742147 Version 1 (Component)
sam5 (coumarate hydroxylase) with native rbs.

BBa_K861101
BBa_K861101 Version 1 (Component)
BcsA with RBS and terminator

BBa_K861111
BBa_K861111 Version 1 (Component)
BcsB with RBS and terminator

BBa_K861121
BBa_K861121 Version 1 (Component)
BcsZ with RBS and terminator

BBa_K861131
BBa_K861131 Version 1 (Component)
BcsC with RBS and terminator

BBa_K315004
BBa_K315004 Version 1 (Component)
variant forward lox site with 3 base changes in the spacer region (loxm2 forward)

BBa_I756021
BBa_I756021 Version 1 (Component)
CMV promoter with Lambda Operator

BBa_I756017
BBa_I756017 Version 1 (Component)
U6 promoter with tet operators

BBa_K906011
BBa_K906011 Version 1 (Component)
Spacer with MluI and SacII

BBa_K1033282
BBa_K1033282 Version 1 (Component)
amilCP blue chromoprotein with strong promoter

BBa_K914012
BBa_K914012 Version 1 (Component)
pSB1A2 with 1 amber mutation

BBa_K265011
BBa_K265011 Version 1 (Component)
OmpA with proteolytic cleavage tag

BBa_K1321302
BBa_K1321302 Version 1 (Component)
pSEVA331-BB with J23100-RFP

BBa_K1403005
BBa_K1403005 Version 1 (Component)
JMT CDS with His Tag

BBa_K771203
BBa_K771203 Version 1 (Component)
VioE with Membrane Anchor 4

BBa_K175003
BBa_K175003 Version 1 (Component)
R751 plasmid with trbK knockout

BBa_I716317
BBa_I716317 Version 1 (Component)
H-NOX with Bca1092 terminator

BBa_K1321304
BBa_K1321304 Version 1 (Component)
pSEVA321-BB with J23117-RFP

BBa_K771202
BBa_K771202 Version 1 (Component)
VioB with Membrane Anchor 3

BBa_K660301
BBa_K660301 Version 1 (Component)
Phosphodiesterase enzyme with 6xHIS tag

BBa_K762101
BBa_K762101 Version 1 (Component)
LIMS1 with embedded stop codon

BBa_K771306
BBa_K771306 Version 1 (Component)
FabG with Membrane Anchor 3

BBa_K1648006
BBa_K1648006 Version 1 (Component)
Insertion kit with GFP-nanobody

BBa_K764012
BBa_K764012 Version 1 (Component)
WbbC with point mutation + BBa_B0014

psb1C3_Int
BBa_K743010 Version 1 (Component)
psb1C3_IntS recombination plasmid with RFP

BBa_K771307
BBa_K771307 Version 1 (Component)
FabZ with Membrane Anchor 4

BBa_K660001
BBa_K660001 Version 1 (Component)
LOV2 Domain with 6xHIS tag

BBa_K1230002
BBa_K1230002 Version 1 (Component)
YFP generator with LsrA promoter, with terminator as a prefix

BBa_K1499205
BBa_K1499205 Version 1 (Component)
MntH with amber stop codon with promoter for expression, no tRNA

BBa_J70597
BBa_J70597 Version 1 (Component)
J70577 cut with BsaXI and with 6his inserts (J70564-fi) added

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.