BBa_J119094BBa_J119094 Version 1 (Component)pT7-RBS-RFP-pLac+tRNA CCAAU+pLac+tRNA CUAGC+pLac+tRNA CUACU
BBa_J119099BBa_J119099 Version 1 (Component)pT7-RBS-RFP-pLac+tRNA CCACC+pLac+tRNA AGGAC+pLac+tRNA CUACC
BBa_J119097BBa_J119097 Version 1 (Component)pT7-RBS-RFP-pLac+tRNA CCACC+pLac+tRNA CUAGC+pLac+tRNA CUACC
BBa_J119098BBa_J119098 Version 1 (Component)pT7-RBS-RFP-pLac+tRNA CCACC+pLac+tRNA CUAGC+pLac+tRNA CUACU
BBa_K1638039BBa_K1638039 Version 1 (Component)Hfq fused to T25 domain of CyaA with cAMP-induced RFP reporter
BBa_K606068BBa_K606068 Version 1 (Component)pVeg SpoVG cI SpoVG RFP Term for B. subtilis and E. coli
BBa_K1638038BBa_K1638038 Version 1 (Component)Yhbj fused to T25 domain of CyaA with cAMP-induced RFP reporter
BBa_K1725103BBa_K1725103 Version 1 (Component)SrpR repressible promoter + strong RBS + RFP + terminator + PhlF repressible promoter + weak RBS + G
BBa_S03675BBa_S03675 Version 1 (Component)RFP<sub>rev</sub>-RBS<sub>rev</sub>-HixC : RBS-TetF-HixC
BBa_K541800BBa_K541800 Version 1 (Component)B.subtilis and E.coli Episomal Shuttle Vector with Consitutive RFP to express in E.coli
tuned pArsBBa_K935002 Version 1 (Component)E. coli ArsR under the control of pArs coupled with pArs + RFP
BBa_J23120BBa_J23120 Version 1 (Component)Locked RFP (J23077) with OriT (J01003). Lock that can be transferred via conjugation
BBa_K199194BBa_K199194 Version 1 (Component)TT-pLux-RBS-LuxI 2 sites-RBS-GFP-RBS-RFP-pLacI-RBS-LuxR
BBa_K2082232BBa_K2082232 Version 1 (Component)RFP under the control of an optimized lacZ promoter combined with Gal11P:cI(434)
BBa_K199193BBa_K199193 Version 1 (Component)TT-pLux-RBS-LuxI 2 sites-RBS-RFP-RBS-GFP-pBAD-RBS-LuxR
BBa_K886002BBa_K886002 Version 1 (Component)Recombination Device composed by Cre-lox71
BBa_K362051BBa_K362051 Version 1 (Component)ahpC promoter - RBS - GFP - Term - Term - sufA promoter - RBS - RFP - Term - Term
BBa_K362052BBa_K362052 Version 1 (Component)ahpC promoter - RBS - RFP - Term - Term - sufA promoter - RBS - GFP - Term - Term
BBa_K092700BBa_K092700 Version 1 (Component)TetR cds with RBS and Terminator + pTetR, RFP with RBS + LuxI with RBS and terminator
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
BBa_K1899000BBa_K1899000 Version 1 (Component)<i>tetp</i>-BFP-<i>phlFp</i>-RFP-<i>lacp</i>-GFP
BBa_K1051351BBa_K1051351 Version 1 (Component)K1051301 (clb2 promoter) + K1051000 (non stop codon RFP) + K1051008 (Stop codon + CAA terminator)
tar-tsrBBa_J22010 Version 1 (Component)Lac promoter-Tar Receptor-CFP reporter-Tet promoter-Tsr receptor-RFP reporter
BBa_K1520521BBa_K1520521 Version 1 (Component)PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter-Plac-tetR-Ter-Pcons2-lacI-Ter
BBa_K1051352BBa_K1051352 Version 1 (Component)K1051302 (clb5 promoter) + K1051000 (non stop codon RFP) + K1051008 (Stop codon + CAA terminator)
BBa_K1051353BBa_K1051353 Version 1 (Component)K1051303 (clb6 promoter) + K1051000 (non stop codon RFP) + K1051008 (Stop codon + CAA terminator)
BBa_K1051350BBa_K1051350 Version 1 (Component)K1051300 (cln2 promoter) + K1051000 (non stop codon RFP) + K1051008 (Stop codon + CAA terminator)
BBa_K1051354BBa_K1051354 Version 1 (Component)K1051304 (cln3 promoter) + K1051000 (non stop codon RFP) + K1051008 (Stop codon + CAA terminator)
BBa_J119125BBa_J119125 Version 1 (Component)Ptrp+(rbs-mRFP) (J119123 ligated to S04419)
BBa_K1650007BBa_K1650007 Version 1 (Component)Composite carrying the Lac-inducible CDI-operon, Lac-inducible RFP and LacI
BBa_K283035BBa_K283035 Version 1 (Component)RFP-double terminators
BBa_K235034BBa_K235034 Version 1 (Component)[K235033][J04450] (constituitive promoter+32??C RBS thermometer+LacI+stop+pLac+RBS+RFP+stop)
PL GFPBBa_K193000 Version 1 (Component)GFP reporter regulated by CI.
BBa_K1767005BBa_K1767005 Version 1 (Component)P(Lac)IQ RBS LuxR ter ter luxpR RBS tetR ter ter P(tetR) RBS mRFP1 ter ter
BBa_S03684BBa_S03684 Version 1 (Component)(1,2) RFP<sub>rev</sub>-RBS<sub>rev</sub>-HixC-pLac-HixC : RBS-TetF-HixC
BBa_J3109BBa_J3109 Version 1 (Component)RFP Reverse
BBa_K1638030BBa_K1638030 Version 1 (Component)Leucine zipper fused to T25 domain of <i>cyaA</i> with cAMP-induced RFP generator
BBa_K1638031BBa_K1638031 Version 1 (Component)Leucine zipper fused to T18 domain of <i>cyaA</i> with cAMP-induced RFP generator
BBa_K1132037BBa_K1132037 Version 1 (Component)AND-inverted RFP gate (BBa_K1132034) with T7 polymerase under the control of a strong promoter
BBa_K1132038BBa_K1132038 Version 1 (Component)XOR-inverted RFP gate (BBa_K1132035) with T7 polymerase under the control of a strong promoter
BBa_K2082231BBa_K2082231 Version 1 (Component)RFP under the control of an optimized lacZ promoter combined with the protein SH2:cI434
BBa_K606012BBa_K606012 Version 1 (Component)RFP mCherry with double terminator
pTetR-LacIBBa_I763030 Version 1 (Component)LacI coding device regulated by pTetR
BBa_K1520021BBa_K1520021 Version 1 (Component)Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-Ter
BBa_S03676BBa_S03676 Version 1 (Component)RFP<sub>rev</sub>-RBS<sub>rev</sub>-HixC : TetB-RBS<sub>rev</sub>-HixC
BBa_K1767003BBa_K1767003 Version 1 (Component)P(Lac)IQ RBS Aiia RBS LuxR ter ter luxpR RBS tetR ter ter P(tetR) RBS mRFP1 ter ter