BBa_K1650007

BBa_K1650007 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1650007
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daniel Stukenberg
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-18 11:02:22

Composite carrying the Lac-inducible CDI-operon, Lac-inducible RFP and LacI



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1650007_sequence (Version 1)

Description

The composite part contains a lac-inducible CDI operon which gives your strain the capability to inhibit growth of bacterial cells that express BamA in their outer membrane (most gram negative bacteria). Additionally the part contains mRFP1 (BBa_E1010) driven by another lac-inducible promoter as induction control. Moreover the composite part contains LacI.
IPTG-induced cells harboring BBa_K1650007 express CdiB which forms a beta-barrel structure. The CdiA protein is transported through CdiB and is displayed on the cell surface. When getting in contact with BamA of a target cell, the C-terminal domain is delivered into the target cell acting as pore forming protein thus destroying the proton motoric force of the target cell.
BBa_K1650007 could be used to improve transformation efficiency. Induced CDI+ cells would kill false positive cells.

Notes

BBa_K1650007 was toxic for cells in pSB1C3 backbone, presumably because of the high metabolic burden. In accordance with iGEM Headquarter, we submitted BBa_K1650007 in the low copy pSB4C5 backbone

Source

The CDI-operon was PCR amplified from genomic DNA of E.coli strain EC93. Other parts were taken from the registry.

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1650007/1