BBa_K1899000

BBa_K1899000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1899000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: iGEM16_HKUST

Date created: 2016-10-17 11:00:00

Date modified: 2016-10-18 05:38:39

tetp-BFP-phlFp-RFP-lacp-GFP

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K1899000_sequence (Version 1)

Description

It is the main construct which is expected to perform as a tristable switch with three discrete, but alternating, steady states. By applying different inducers, three repressible promoters would act accordingly, producing three different outputs.

Notes

NIL

Source

Most of the parts come from the Part Registry, except for the BBa_K1899004, sequence of which is obtained from the paper, Genomic mining of prokaryotic repressors for orthogonal logic gates.

Components

Access	Instance	Definition
public	BBa_R0040	p(tetR)
public	BBa_S05326	BBa_S05326
public	BBa_K1899009	BBa_K1899009
public	BBa_K1899001	BBa_K1899001
public	BBa_K1899004	BBa_K1899004
public	BBa_K1899009	BBa_K1899009
public	BBa_K1899010	BBa_K1899010
public	BBa_K516032	BBa_K516032
public	BBa_R0010	LacI
public	BBa_K1899010	BBa_K1899010
public	BBa_S05326	BBa_S05326
public	BBa_K1899003	BBa_K1899003

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0040	1,54	p(tetR)
BBa_S05326	63,684	BBa_S05326
BBa_K1899009	693,1864	BBa_K1899009
BBa_K1899001	1873,2643	BBa_K1899001
BBa_K1899004	2652,2717	BBa_K1899004
BBa_K1899009	2726,3897	BBa_K1899009
BBa_K1899010	3906,4609	BBa_K1899010
BBa_K516032	4618,5479	BBa_K516032
BBa_R0010	5488,5687	LacI
BBa_K1899010	5696,6399	BBa_K1899010
BBa_S05326	6408,7029	BBa_S05326
BBa_K1899003	7038,7891	BBa_K1899003

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• igem#status: Planning
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_K1899000/1

Member of these Collections

• iGEM Parts Registry