BBa_K175046
BBa_K175046 Version 1 (Component)
Plasmid 1 for Transcriptional Cascade subproject TU Delft iGEM 09

BBa_J48104
BBa_J48104 Version 1 (Component)
NikR promoter, a protein of the ribbon helix-helix family of trancription factors that repress expre

BBa_K1368008
BBa_K1368008 Version 1 (Component)
mcherry generator driven by HSL under the post transcriptional control

BBa_K175047
BBa_K175047 Version 1 (Component)
Plasmid 2 for Transcriptional Cascade subproject TU Delft iGEM 09

BBa_K199153
BBa_K199153 Version 1 (Component)
Measures the reverse activity of pLux in absence of LuxR

BBa_K199154
BBa_K199154 Version 1 (Component)
Measures the reverse activity of pLux in absence of LuxR

BBa_K199152
BBa_K199152 Version 1 (Component)
Measures the reverse activity of pLux in the presence of LuxR

BBa_K391001
BBa_K391001 Version 1 (Component)
Construct for characterization of P2 activity in the presence of AIP.

BBa_K391000
BBa_K391000 Version 1 (Component)
Construct for characterization of P2 activity in the presence of AIP.

BBa_M36291
BBa_M36291 Version 1 (Component)
Transcription terminator (apFAB391) (99% efficient)

BBa_M36305
BBa_M36305 Version 1 (Component)
Transcription terminator pT-T7 from bacteriophage

BBa_J37017
BBa_J37017 Version 1 (Component)
AiiA Expression Assay (can be used in conjunction with AHL assay to detect activity)

BBa_K880001
BBa_K880001 Version 1 (Component)
Asymmetrically digestible reporter to assay the activity of DNA recombinases FimE K137007 and HbiF K

BBa_K346078
BBa_K346078 Version 1 (Component)
Constutitive promoter BBa_J23109+pag activator(BBa_I746352)

BBa_K136041
BBa_K136041 Version 1 (Component)
Strongest RBS - lasR activator with LVA

CeaB
BBa_K131004 Version 1 (Component)
Colicin E2 Activity Gene (CeaB)

BBa_K944001
BBa_K944001 Version 1 (Component)
Transcriptional Regulator for Cyanide Inducible Promoter

BBa_K2092004
BBa_K2092004 Version 1 (Component)
alcR (incl RBS), ethanol-activated transcription factor from A. nidulans

BBa_K1320000
BBa_K1320000 Version 1 (Component)
Cd sensitive promoter with Ogr activator, PO promoter, and GFP

BBa_K1968014
BBa_K1968014 Version 1 (Component)
Tcyc Phytobrick: cytochrome C gene transcriptional terminator from Saccharomyces cerevisiae

Bacillus subtilis Collection
bsu_collection Version 1 (Collection)
This collection includes information about promoters, operators, CDSs and proteins from Bacillus subtilis. Functional interactions such as transcriptional activation and repression, protein production and various protein-protein interactions are also included.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.