GG98
BBa_K2145105 Version 1 (Component)
GFP/RFP reporter (Reverse/Reverse) with random 500bp spacer

GG102
BBa_K2145127 Version 1 (Component)
GFP/RFP reporter (Reverse/Reverse) with random 500bp spacer

BBa_M1507
BBa_M1507 Version 1 (Component)
Protein used to add two hydroxyl groups to pyrene

C2HP/RGE
BBa_K1442107 Version 1 (Component)
C2HP Testing Module (reverse GFP)

BBa_K541800
BBa_K541800 Version 1 (Component)
B.subtilis and E.coli Episomal Shuttle Vector with Consitutive RFP to express in E.coli

BBa_K084003
BBa_K084003 Version 1 (Component)
3OC6HSL -> Venus YFP

BBa_K1401610
BBa_K1401610 Version 1 (Component)
pLac - BCD2 - YFP

pmyo2-ChR2
BBa_K1634007 Version 1 (Component)
pmyo2-ChR2-YFP

BBa_K592032
BBa_K592032 Version 1 (Component)
J23101-B0032-YFP

BBa_J85001
BBa_J85001 Version 1 (Component)
inducible RFP, constitutive GFP, terminators after each gene

rev RFP
BBa_K1463520 Version 1 (Component)
E1010 RFP in reverse

BBa_K797012
BBa_K797012 Version 1 (Component)
Lactose induced GFP and RFP generator asembled by Golden gate assembly

IBMc322
IBMc322 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-GCS-mCherry*(193-236)-H6-Ter

IBMc326
IBMc326 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-5)-BsaI-BsaI-ECF20(187-193)-Ter

IBMc461
IBMc461 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B32-bla(1-28)-BsaI-BsaI-bla(285-286)-Ter

IBMc323
IBMc323 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-GAEYCFNS-mCherry*(193-236)-H6-Ter

IBMc227
IBMc227 Version 1 (Component)
pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR(1-20)-BsaI-BsaI-TetR(188-207)-Ter

IBMc333
IBMc333 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-SrpR(1-22)-BsaI-BsaI-SrpR(192-213)-Ter

BBa_K1212020
BBa_K1212020 Version 1 (Component)
TBS2+RBS+GFP+T

BclA-His
BBa_K1933000 Version 1 (Component)
N-terminal domain of BclA protein fused to 6xHis tag

BBa_K2066125
BBa_K2066125 Version 1 (Component)
To be completed

YFP_SPtom4
BBa_K809316 Version 1 (Component)
YFP + signal peptide of Tom40

BBa_J24676
BBa_J24676 Version 1 (Component)
pBAD to tetR coding region, lacI coding region, and mCherry reporter

BBa_K584001
BBa_K584001 Version 1 (Component)
Constitutive promoter + GFP generator

BBa_K371020
BBa_K371020 Version 1 (Component)
RFP[intermediate]

BBa_K577003
BBa_K577003 Version 1 (Component)
YFP Coding Device

BBa_K199021
BBa_K199021 Version 1 (Component)
RBS-RFP (all inverted)

BBa_J01031
BBa_J01031 Version 1 (Component)
[OnLock??][spo0A][DblTerminator][pspoIIE YFP]

BBa_K2188090
BBa_K2188090 Version 1 (Component)
CAG promoter and YFP

BBa_J06005
BBa_J06005 Version 1 (Component)
YFP on LuxBox

Little Bub
BBa_J06105 Version 1 (Component)
YFP on LasBox

Prm+RFP
BBa_S03337 Version 1 (Component)
Prm+ with RFP as a reporter

Pr+CFP
BBa_S03464 Version 1 (Component)
Pr with CFP as a reporter

BBa_K1321112
BBa_K1321112 Version 1 (Component)
Phytochelatin (PC) EC20 fused to CBDcex with LacI promoter

BBa_K1321105
BBa_K1321105 Version 1 (Component)
CBDcex fused to Phytochelatin (PC) EC20 with T7 promoter

BBa_K1321110
BBa_K1321110 Version 1 (Component)
Phytochelatin (PC) EC20 fused to linker-dCBD with LacI

BBa_K1321115
BBa_K1321115 Version 1 (Component)
CBDcex fused to phytochelatin (PC) EC20 with LacI promoter

BBa_K1321114
BBa_K1321114 Version 1 (Component)
CBDclos fused to phytochelatin (PC) EC20 with LacI promoter

BBa_K1321101
BBa_K1321101 Version 1 (Component)
Phytochelatin (PC) EC20 fused to CBDclos with T7 promoter

BBa_K1321104
BBa_K1321104 Version 1 (Component)
CBDclos fused to Phytochelatin (PC) EC20 with T7 promoter

BBa_K575011
BBa_K575011 Version 1 (Component)
LasR/PAI1 Inducible Promoter + RBS (B0034) + RFP

BBa_K194001
BBa_K194001 Version 1 (Component)
GFP (a yeast- and FACS-optimized GFP variant),

BBa_K201999
BBa_K201999 Version 1 (Component)
To be Deleted

pSB3K3
pSB3K3 Version 1 (Component)
Low to medium copy BioBrick standard vector

Plamda-GFP
BBa_I763011 Version 1 (Component)
promoter lambda (cI regulated) with GFP (+LVA) reporter

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1053206
BBa_K1053206 Version 1 (Component)
Pconst.- RBS- YF1/FixJ-DT- Pfixk2- HHR*- GFP- DT

YF1-HHR
BBa_K1053205 Version 1 (Component)
Pconst. -RBS- YF1/FixJ-DT- Pfixk2- HHR- GFP- DT

BBa_K741003
BBa_K741003 Version 1 (Component)
pRM-RBS-GFP-T

CspB | RFP
BBa_K525234 Version 1 (Component)
Fusion Protein of mRFP, S-layer cspB from Corynebacterium halotolerans with TAT-sequence, PT7, RBS