BBa_K876016BBa_K876016 Version 1 (Component)plac-lacO-RBS-GFP-T-pTet-LacI
BBa_K091190BBa_K091190 Version 1 (Component)plux/cI+RBS+lasI+RBS+Mnt+TT+pMnt/lac+RBS+lasI+RBS+cI+TT
pTetR-LacIBBa_I763030 Version 1 (Component)LacI coding device regulated by pTetR
BBa_K2139009BBa_K2139009 Version 1 (Component)Ptac RBS Endo5a
BBa_K079024BBa_K079024 Version 1 (Component)LacI repressor under the control of the J23118 costitutive promoter and Lac 1 operator
BBa_J36849BBa_J36849 Version 1 (Component)Lac-inducible generator of Lpp-OmpA(46-66)-Streptavidin single-chain dimeric + His6 tag
BBa_J36851BBa_J36851 Version 1 (Component)Lac-inducible generator of Lpp-OmpA(46-159)-Streptavidin single-chain dimeric + His6 tag
BBa_K077041BBa_K077041 Version 1 (Component)AiiA and cII under control of plac promotor
BBa_K831014BBa_K831014 Version 1 (Component)Inducible istR (inhibitor of SOS-induced toxicity by RNA) under the control of lac promoter
BBa_K299029BBa_K299029 Version 1 (Component)RBS measurement device pT7 J61127 GFP
BBa_K639004BBa_K639004 Version 1 (Component)rrnB P1-LacI-pLac-mCherry plausible stress sensor
BBa_K843003BBa_K843003 Version 1 (Component)Lambda cI regulated by PLacI
BBa_M45760BBa_M45760 Version 1 (Component)K208010:M45710, A composite part for AvrA with a Lac promoter and ribosome binding site.
BBa_K079052BBa_K079052 Version 1 (Component)LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac symmetric operato
BBa_K079020BBa_K079020 Version 1 (Component)GFP reporter under the control of J23118 promoter and Lac 2 operator auto-regulated by LacI protein
BBa_K079051BBa_K079051 Version 1 (Component)LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac 1 operator
BBa_I763003BBa_I763003 Version 1 (Component)GFP coding device switched on by IPTG
BBa_K2036028BBa_K2036028 Version 1 (Component)placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-ompA-iLDH-TT-pRM-RBS-beta-galactosidase
BBa_K1767005BBa_K1767005 Version 1 (Component)P(Lac)IQ RBS LuxR ter ter luxpR RBS tetR ter ter P(tetR) RBS mRFP1 ter ter
BBa_S03737BBa_S03737 Version 1 (Component)pLac-lox-RFP(reverse)-TT-lox-RBS-Tet (psB1A2)
pTetR-RFP-BBa_I763040 Version 1 (Component)RFP regulated by TetR and GFP regulated by pLac
BBa_K2036024BBa_K2036024 Version 1 (Component)placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-iLDH-TT-pRM-RBS-beta-galactosidase
BBa_K1813002BBa_K1813002 Version 1 (Component)Lac I repressor
BBa_K1606010BBa_K1606010 Version 1 (Component)Kumamax enzyme that gluten updated m-cherry reporter device
BBa_K511905BBa_K511905 Version 1 (Component)Repressible rtTA3 Transactivator Generator (Hef1a-LacO-rtTA3) MammoBlock Device
BBa_K1767003BBa_K1767003 Version 1 (Component)P(Lac)IQ RBS Aiia RBS LuxR ter ter luxpR RBS tetR ter ter P(tetR) RBS mRFP1 ter ter
BBa_K1341011BBa_K1341011 Version 1 (Component)OR LOGIC GATE IN Graph Theory (GFP OUTPUT DEVICE)
BBa_J119075BBa_J119075 Version 1 (Component)pT7-RBS-RFP-pLacI-RBS-T7RNAP
BBa_K358000BBa_K358000 Version 1 (Component)Lac promoter with GFP
BBa_K137033BBa_K137033 Version 1 (Component)Device with GFP with (AC)21 repeat after start codon
CP1LacPI2BBa_K418002 Version 1 (Component)IPTG inducible Lac promoter cassette
pCMV-ECFP-BBa_I763023 Version 1 (Component)LacI coding device with ECFP as a reporter regulated by pCMV
BBa_J04480BBa_J04480 Version 1 (Component)EYFP coding device with promoter, RBS, and terminator. No degradation tag.
BBa_J04431BBa_J04431 Version 1 (Component)GFP Coding Device with promoter, RBS, GFP with LVA tag, and Terminator
BBa_I715071BBa_I715071 Version 1 (Component)Lac promoter with Ribosomal Binding Site
BBa_K2123117BBa_K2123117 Version 1 (Component)Novel RFP device regulated by mercury: MerR (regulatory protein) + Stationary phase with mer operato
BBa_K590046BBa_K590046 Version 1 (Component)AAR-PSB3K3-Lac Inducible w/o LacI
BBa_K418001BBa_K418001 Version 1 (Component)From partsregistry.org IPTG inducible Lac promoter cassette
BBa_K1520509BBa_K1520509 Version 1 (Component)PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter
BBa_K584011BBa_K584011 Version 1 (Component)Lac-Lux hybrid promotor + CrtEBI + CI repressor + INP
Adapter BiBBa_K1807015 Version 1 (Component)This device allows for the IPTG-inducible expression of lacZα peptide which in the presence of
BBa_K1222004BBa_K1222004 Version 1 (Component)pCam(T7 promoter+lac operator+CamR antisense 2+T7 terminator)
BBa_K726009BBa_K726009 Version 1 (Component)T7 driven lac operated inducer for the rhl quorum-sensing system
BBa_J22121BBa_J22121 Version 1 (Component)Lac Y gene under the rec A(SOS) promoter in plasmid pSB2K3
iGEM Parts Registryigem_collection Version 1 (Collection)The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.