pTetR-RFP-

BBa_I763040 Version 1

Component

This part has been discontinued.

Please consider using BBa_I763034 as a replacement.

Source: http://parts.igem.org/Part:BBa_I763040

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Alice Pasini

Date created: 2007-10-21 11:00:00

Date modified: 2015-08-31 04:08:09

RFP regulated by TetR and GFP regulated by pLac

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_I763040_sequence (Version 1)

Description

Plac activity Tester with a GFP as a reporter. The constitutive promoter pTeTR regulates the expression of LacI inibitor in order to avoid undesired pLac promoter activation due to unsufficient endogenous LacI.

Notes

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Source

Igem 2007 plates

Components

Access	Instance	Definition
public	BBa_R0040	p(tetR)
public	BBa_B0034	BBa_B0034
public	BBa_E1010	mRFP1
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012
public	BBa_R0010	LacI
public	BBa_B0034	BBa_B0034
public	BBa_J04031	BBa_J04031
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0040	1,54	p(tetR)
BBa_B0034	63,74	BBa_B0034
BBa_E1010	81,761	mRFP1
BBa_B0010	795,874	BBa_B0010
BBa_B0012	883,923	BBa_B0012
BBa_R0010	932,1131	LacI
BBa_B0034	1140,1151	BBa_B0034
BBa_J04031	1158,1916	BBa_J04031
BBa_B0010	1925,2004	BBa_B0010
BBa_B0012	2013,2053	BBa_B0012

Other Properties

• igem#experience: Works
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• isReplacedBy: BBa_I763034/1
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I763040/1

Member of these Collections

• iGEM Parts Registry