BBa_K908016BBa_K908016 Version 1 (Component)Microcin B17, Gene B flanked by Attc sequence and rbs
BBa_K908017BBa_K908017 Version 1 (Component)Microcin B17, Gene C flanked by Attc sequence and rbs
BBa_K908018BBa_K908018 Version 1 (Component)Microcin B17, Gene F flanked by Attc sequence and rbs
BBa_K908019BBa_K908019 Version 1 (Component)Microcin C7, Gene E flanked by Attc sequence and rbs
BBa_K908020BBa_K908020 Version 1 (Component)Microcin C7, Gene C flanked by Attc sequence and rbs
pLac ColE2BBa_K914001 Version 1 (Component)pLac Colicin E2 Immunity protein
BBa_K908021BBa_K908021 Version 1 (Component)Microcin C7, Gene F flanked by Attc sequence and rbs
BBa_K914002BBa_K914002 Version 1 (Component)repressilator RBS Colicin E2 Immunity protein
BBa_J72218BBa_J72218 Version 1 (Component)kanamycin resistant p15A vector
BBa_K782016BBa_K782016 Version 1 (Component)10x[TALA+TALB] operator_CMV promoter_mCitrine
BBa_K782017BBa_K782017 Version 1 (Component)10x[TALA] operator_CMV promoter_mCitrine
BBa_K782018BBa_K782018 Version 1 (Component)10x[TALB] operator_CMV promoter_mCitrine
BBa_K782019BBa_K782019 Version 1 (Component)10x[TALA+TALB] operator_CMV promoter_fLuciferase
BBa_K782020BBa_K782020 Version 1 (Component)10x[TALA] operator_CMV promoter_fLuciferase
BBa_K782021BBa_K782021 Version 1 (Component)10x[TALB] operator_CMV promoter_fLuciferase
BBa_K782022BBa_K782022 Version 1 (Component)12x[TALD] operator_CMV promoter_fLuciferase
BBa_K782023BBa_K782023 Version 1 (Component)12x[NicTAL] operator_CMV promoter_fLuciferase
BBa_K782024BBa_K782024 Version 1 (Component)2x[NicTAL]+2x[TALD] operator_minimal promoter_mCitrine
BBa_K782025BBa_K782025 Version 1 (Component)4x[NicTAL]+4x[TALD] operator_minimal promoter_mCitrine
BBa_K782026BBa_K782026 Version 1 (Component)7x[TALA+TALB] operator_minimal promoter_mCitrine
BBa_K782027BBa_K782027 Version 1 (Component)7x[TALA] operator_minimal promoter_mCitrine
BBa_K782028BBa_K782028 Version 1 (Component)10x[TALA+TALB] operator_minimal promoter_mCitrine
BBa_K782029BBa_K782029 Version 1 (Component)10x[TALA] operator_minimal promoter_mCitrine
BBa_S04140BBa_S04140 Version 1 (Component)S04139:B0015
Bxb1_XisBBa_K907001 Version 1 (Component)Mycobacterium Phage Bxb1 gp47, DNA excisionase
BBa_K782030BBa_K782030 Version 1 (Component)10x[TALB] operator_minimal promoter_mCitrine
BBa_K782031BBa_K782031 Version 1 (Component)12x[TALD] operator_minimal promoter_mCitrine
BBa_K782032BBa_K782032 Version 1 (Component)10x[TALA+TALB] operator_minimal promoter_fLuciferase
BBa_K782033BBa_K782033 Version 1 (Component)10x[TALA] operator_minimal promoter_fLuciferase
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
Cello E. Coli PartsEco1C1G1T1_collection Version 1 (Collection)These are the Cello parts for E. Coli circuits
BBa_I730002BBa_I730002 Version 1 (Component)pLacI-tetR repressor
BBa_K098997BBa_K098997 Version 1 (Component)cIts coding region
BBa_K098998BBa_K098998 Version 1 (Component)cIts coding region with rbs
BBa_S04136BBa_S04136 Version 1 (Component)terminator and cI promoter
BBa_S04137BBa_S04137 Version 1 (Component)heat sensitive cI QPI
BBa_K098995BBa_K098995 Version 1 (Component)heat sensitive cI QPI with high promoter
BBa_K098993BBa_K098993 Version 1 (Component)heat sensitive cI QPI with low promoter
BBa_K098991BBa_K098991 Version 1 (Component)cI promoter with GFP reporter
BBa_K105021BBa_K105021 Version 1 (Component)cI - operator
BBa_S04138BBa_S04138 Version 1 (Component)cI QPI with GFP reporter system
BBa_K098988BBa_K098988 Version 1 (Component)temperature sensitive cI inducible system with GFP reporter and high promoter
BBa_K098987BBa_K098987 Version 1 (Component)temperature sensitive cI inducible system with GFP reporter and low promoter
BBa_K098984BBa_K098984 Version 1 (Component)lac inducible mtrB (high expression of QPI)
BBa_K098982BBa_K098982 Version 1 (Component)lac inducible system with low promoter and GFP reporter
BBa_K098983BBa_K098983 Version 1 (Component)lac inducible mtrB (low expression of QPI)
BBa_K157010BBa_K157010 Version 1 (Component)glycine-serine linker fused to B-cell receptor transmembrane region; displays protein on cell surfac
Tm-Split-LBBa_K157020 Version 1 (Component)Tm-Split-Luciferase1/2
cI 434BBa_R0052 Version 1 (Component)Promoter (434 cI regulated)