BBa_K2044022
BBa_K2044022 Version 1 (Component)
Based on our project, it represents Site NO.8 in the map.

BBa_K2044019
BBa_K2044019 Version 1 (Component)
Based on our project, it represents Site NO.5 in the map.

BBa_K180005
BBa_K180005 Version 1 (Component)
GoL - Primary plasmid (part 1)/RPS - Paper primary plasmid (part 1) [LuxR generator]

BBa_I731014
BBa_I731014 Version 1 (Component)
The luxR based receiver, F2620 (formerly I13270), controls the production of mCherry

BBa_K987000
BBa_K987000 Version 1 (Component)
This part is a coding part that produces Vip3Ca3, a protein that can deals with different forms of p

BBa_K1638035
BBa_K1638035 Version 1 (Component)
hTrx-based scaffold fused to T18 through intein and a flexible linker

BBa_M50053
BBa_M50053 Version 1 (Component)
FRET-based glucose sensor using a glucose binding protein, mRuby3 and cometGFP

BBa_M1722
BBa_M1722 Version 1 (Component)
Part is based on theory. Consists of pBAD promoter, hepcidin and GFP

BBa_K187254
BBa_K187254 Version 1 (Component)
folD, ORF, forward primer

BBa_K750008
BBa_K750008 Version 1 (Component)
Quorum sensing system based on LuxI and LuxR to control the expression of parts behind

BBa_K185033
BBa_K185033 Version 1 (Component)
An inverter of a special lactose operon system based on J23110-rbs34-lacI-dter-plac-rbs31

BBa_K2044001
BBa_K2044001 Version 1 (Component)
Based on our project, <2-4-8> is a feasible pathway from Site No. 2 to Site No. 8

BBa_K2044000
BBa_K2044000 Version 1 (Component)
Based on our project, <2-6-8> is the optimal pathway scheme from Site No. 2 to Site No. 8

BBa_K2044010
BBa_K2044010 Version 1 (Component)
Based on our project, <5,7> is the direct pathway from Site No.5to Site No.7 in the map we design.

BBa_K371054
BBa_K371054 Version 1 (Component)
MPF(meta-prefix)+[GFP+10*GS+A] fusion protein+MSF(meta-suffix))

BBa_K2044006
BBa_K2044006 Version 1 (Component)
Based on our project,<3,6> is the direct pathway from Site No.3 to Site No.6 in the map we design.

BBa_K2044004
BBa_K2044004 Version 1 (Component)
Based on our project, <2,4> is the direct pathway from Site No.2 to Site No.4 in the map we design.

BBa_K2044009
BBa_K2044009 Version 1 (Component)
Based on our project, <4,8> is the direct pathway from Site No.4 to Site No.8 in the map we design.

BBa_K2044014
BBa_K2044014 Version 1 (Component)
Based on our project, <1,4> is the direct pathway from Site No.1 to Site No.4 in the map we design.

BBa_K2044007
BBa_K2044007 Version 1 (Component)
Based on our project, <4,5> is the direct pathway from Site No.4 to Site No.5 in the map we design.

BBa_K2044013
BBa_K2044013 Version 1 (Component)
Based on our project,<7,8> is the direct pathway from Site No.7 to Site No.8 in the map we design.

BBa_K2044008
BBa_K2044008 Version 1 (Component)
Based on our project, <4,6> is the direct pathway from Site No.4 to Site No.6 in the map we design.

BBa_K2044003
BBa_K2044003 Version 1 (Component)
Based on our project, <2,3> is the direct pathway from Site No.2 to Site No.3 in the map we design.

BBa_K2044002
BBa_K2044002 Version 1 (Component)
Based on our project, <2,1> is the direct pathway from Site No.2 to Site No.1 in the map we design.

BBa_K2044005
BBa_K2044005 Version 1 (Component)
Based on our project, <2,6> is the direct pathway from Site No.2 to Site No.6 in the map we design.

BBa_K2044012
BBa_K2044012 Version 1 (Component)
Based on our project, <6,8> is the direct pathway from Site No.6 to Site No.8 in the map we design.

BBa_K2044011
BBa_K2044011 Version 1 (Component)
Based on our project,<6,4> is the direct pathway from Site No.6 to Site No.4 in the map we design.

BBa_K310012
BBa_K310012 Version 1 (Component)
Composite part of golS with RBS and terminator.

PchiP-lacZ
BBa_K564002 Version 1 (Component)
Chitoporin fused with lacZ - target for sRNA based regulation

BBa_K1520509
BBa_K1520509 Version 1 (Component)
PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.