Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Amanda Urke, Tofe Alimi, Katherine Vera
Date created: 2016-12-11 12:00:00
Date modified: 2016-12-11 10:00:33
FRET-based glucose sensor using a glucose binding protein, mRuby3 and cometGFP
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_M50053_sequence (Version 1) |
Description
This is a device that uses a stable glucose binding protein (GBP) flanked with two compatible fluorescent proteins to indicate glucose levels with a fluorescent resonance energy transfer (FRET) based actuator. The device input is glucose-binding and the device output is fluorescent spectra of the two flurophore. FRET was achieved using mRuby3 as the acceptor fluorophore and cometGFP as the donor fluorophore. Upon glucose binding, this device will undergo a conformational change such that the cometGFP protein in an excited state would transfer energy to the acceptor mRuby3 to be emitted. Quantifying this excitation/emission pair would reveal the extent to which FRET and thus glucose binding that had occurred, relative to the open, unbounded state.Notes
design notesSource
source| Access | Instance | Definition |
| public public public public public public public | BBa_M50031 BBa_M36771 BBa_M50009 BBa_M50036 BBa_M50037 BBa_M50036 BBa_M36936 | BBa_M50031 BBa_M36771 BBa_M50009 BBa_M50036 BBa_M50037 BBa_M50036 BBa_M36936 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_M50031 BBa_M36771 BBa_M50009 BBa_M50036 BBa_M50037 BBa_M50036 BBa_M36936 | 1,48 49,61 62,772 773,790 791,2029 2030,2047 2048,2320 | BBa_M50031 BBa_M36771 BBa_M50009 BBa_M50036 BBa_M50037 BBa_M50036 BBa_M36936 |