BBa_K539281BBa_K539281 Version 1 (Component)rbs+crtZ(beta-carotene hydroxylase) under heat sensitive cI regulater
BBa_K346030BBa_K346030 Version 1 (Component)T7p(BBa_I719005)+RBS(B0034)+DsbA+MBP(periplasm display of lead binding peptide)
7x HisBBa_T2001 Version 1 (Component)7x His tag head domain
BBa_J70606BBa_J70606 Version 1 (Component)J70590 cut with BsaXI and with dead fusion inserts (J70556d-fi,ri) added
BBa_K346029BBa_K346029 Version 1 (Component)T7p(BBa_I719005)+RBS(B0034)+Lpp-OmpA+MBP(surface display of lead binding peptide)
BBa_J70622BBa_J70622 Version 1 (Component)RFC12 cMyc Tag Head Domain
BBa_J70624BBa_J70624 Version 1 (Component)RFC12 FLAG Tag Head Domain
BBa_K2055019BBa_K2055019 Version 1 (Component)Part designed for verification and characterization of the heat the shock promoter and Inverter.
BBa_J24802BBa_J24802 Version 1 (Component)Lead Detector Gene
BBa_I721004BBa_I721004 Version 1 (Component)Lead Binding Protein + Promoter (version a)
BBa_J92001BBa_J92001 Version 1 (Component)Lead Remover and Reporter Device
BBa_K112400BBa_K112400 Version 1 (Component)Promoter for grpE gene - Heat Shock and Ultrasound Sensitive
BBa_K294205BBa_K294205 Version 1 (Component)This is a coding sequence of heat shock protein from E.coli
BBa_K251000BBa_K251000 Version 1 (Component)coding sequene for E.coli heat shock protein hsp15 (example/ european meeting)
BBa_K1613017BBa_K1613017 Version 1 (Component)K1613017 is a new part which can detect lead (Pb) ions in the liquid.
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.