BBa_K2055019

BBa_K2055019 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2055019

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Raul Garza & Carlos Vasquez

Date created: 2016-09-27 11:00:00

Date modified: 2016-09-28 06:02:07

Part designed for verification and characterization of the heat the shock promoter and Inverter.

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K2055019_sequence (Version 1)

Description

We used an existing RBS + RFP from iGEM parts. LacI and FUR proteins are optimized for Acidithiobacillus ferrooxidans. It has two RBS after the constitutive promoter and before the FUR CDS.

Notes

RFP is used as a reporter.

Source

Composite part

Components

Access	Instance	Definition
public	BBa_K2055013	BBa_K2055013
public	BBa_B0034	BBa_B0034
public	BBa_K2055009	BBa_K2055009
public	BBa_K2055010	BBa_K2055010
public	BBa_B0034	BBa_B0034
public	BBa_K2055007	BBa_K2055007
public	BBa_B0012	BBa_B0012
public	BBa_R0011	lacI+pL
public	BBa_B0034	BBa_B0034
public	BBa_E1010	mRFP1
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K2055013	1,89	BBa_K2055013
BBa_B0034	98,109	BBa_B0034
BBa_K2055009	116,595	BBa_K2055009
BBa_K2055010	604,622	BBa_K2055010
BBa_B0034	631,642	BBa_B0034
BBa_K2055007	649,1734	BBa_K2055007
BBa_B0012	1743,1783	BBa_B0012
BBa_R0011	1792,1845	lacI+pL
BBa_B0034	1855,1866	BBa_B0034
BBa_E1010	1873,2578	mRFP1
BBa_B0010	2587,2666	BBa_B0010
BBa_B0012	2675,2715	BBa_B0012

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2055019/1

Member of these Collections

• iGEM Parts Registry